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Title: Genomic organization of the murine CTL lipase gene

Abstract

Murine cytotoxic T-lymphocyte (CTL) lipase was originally identified as an IL-4-inducible gene in CD8-positive T cells. To further our understanding of both the function and the regulation of CTL lipase in T cells, we have cloned and characterized the murine gene. Two overlapping phage clones spanning 29 kb contain the entire CTL lipase gene. The exon structure in similar to those characterized for the human and canine pancreatic lipase-related protein 1 genes, with notable differences in the 5{prime} end. Transcripts initiate from a site that matches a consensus for an initiator sequence. Potential cis-regulatory elements in the CTL lipase 5{prime} regulatory region that would confer dual tissue specificity in exocrine pancreas and cytotoxic T lymphocytes are identified. The implications of this promoter organization are discussed. 27 refs., 2 figs.

Authors:
; ;  [1]
  1. Harvard School of Public Health, Boston, MA (United States)
Publication Date:
OSTI Identifier:
466001
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 35; Journal Issue: 3; Other Information: PBD: 1 Aug 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; LIPASES; STRUCTURE-ACTIVITY RELATIONSHIPS; GENE REGULATION; DNA-CLONING; TRANSCRIPTION; TISSUE DISTRIBUTION; DNA SEQUENCING; SPLICING; MICE; LYMPHOCYTES; POLYMERASE CHAIN REACTION

Citation Formats

Kaplan, M H, Boyer, S N, and Grusby, M J. Genomic organization of the murine CTL lipase gene. United States: N. p., 1996. Web. doi:10.1006/geno.1996.0407.
Kaplan, M H, Boyer, S N, & Grusby, M J. Genomic organization of the murine CTL lipase gene. United States. https://doi.org/10.1006/geno.1996.0407
Kaplan, M H, Boyer, S N, and Grusby, M J. 1996. "Genomic organization of the murine CTL lipase gene". United States. https://doi.org/10.1006/geno.1996.0407.
@article{osti_466001,
title = {Genomic organization of the murine CTL lipase gene},
author = {Kaplan, M H and Boyer, S N and Grusby, M J},
abstractNote = {Murine cytotoxic T-lymphocyte (CTL) lipase was originally identified as an IL-4-inducible gene in CD8-positive T cells. To further our understanding of both the function and the regulation of CTL lipase in T cells, we have cloned and characterized the murine gene. Two overlapping phage clones spanning 29 kb contain the entire CTL lipase gene. The exon structure in similar to those characterized for the human and canine pancreatic lipase-related protein 1 genes, with notable differences in the 5{prime} end. Transcripts initiate from a site that matches a consensus for an initiator sequence. Potential cis-regulatory elements in the CTL lipase 5{prime} regulatory region that would confer dual tissue specificity in exocrine pancreas and cytotoxic T lymphocytes are identified. The implications of this promoter organization are discussed. 27 refs., 2 figs.},
doi = {10.1006/geno.1996.0407},
url = {https://www.osti.gov/biblio/466001}, journal = {Genomics},
number = 3,
volume = 35,
place = {United States},
year = {Thu Aug 01 00:00:00 EDT 1996},
month = {Thu Aug 01 00:00:00 EDT 1996}
}