Characterization and chromosomal localization of the gene for human rhodopsin kinase

Journal Article · Thu Aug 01 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0399· OSTI ID:465994

Khani, S C; Yamamoto, S; Dryja, T P ^[1]

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1. Massachusetts Eye and Ear Infirmary, Boston, MA (United States); and others

G-protein-dependent receptor kinases (GRKs) play a key role in the adapatation of receptors to persistent stimuli. In rod photoreceptors rhodopsin kinase (RK) mediates rapid densensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin. To study the structure and mechanism of FRKs in human photoreceptors, we have isolated and characterized cDNA and genomic clones derived from the human RK locus using a bovine rhodopsin kinase cDNA fragment as a probe. The RK locus, assigned to chromosome 13 band q34, is composed of seven exons that encode a protein 92% identical in amino acid sequence to bovine rhodopsin kinase. The marked difference between the structure of this gene and that of another recently clone human GRK gene suggests the existence of a wide evolutionary gap between members of the GRK gene family. 39 refs., 3 figs.

• OSTI ID: 465994
• Journal Information: Genomics, Vol. 35, Issue 3; Other Information: PBD: 1 Aug 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
PHOSPHOTRANSFERASES
GENETIC MAPPING
BIOLOGICAL EVOLUTION
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
GENE MUTATIONS
DNA-CLONING
HUMAN CHROMOSOME 13
MAN
HEREDITARY DISEASES
SENSE ORGANS DISEASES
RHODOPSIN
PHOSPHORYLATION
PROBES
AMINO ACID SEQUENCE
POLYMERASE CHAIN REACTION
DNA HYBRIDIZATION
NUCLEOTIDES
CATTLE