The gene for human glutaredoxin (GLRX) is localized to human chromosome 5q14

Journal Article · Tue Mar 05 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0141· OSTI ID:459013

Padilla, C A; Holmgren, A ^[1]; Bajalica, S; Lagercrantz, J ^[2]

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1. Karolinska Inst., Stockholm (Sweden)
2. Karolinska Hospital, Stockholm (Sweden)

Glutaredoxin is a small protein (12 kDa) catalyzing glutathione-dependent disulfide oxidoreduction reactions in a coupled system with NADPH, GSH, and glutathione reductase. A cDNA encoding the human glutaredoxin gene (HGMW-approved symbol GLRX) has recently been isolated and cloned from a human fetal spleen cDNA library. The screening of a human fetal spleen cDNA library. The screening of a human genomic library in Charon 4A led to the identification of three genomic clones. Using fluorescence in situ hybridization to metaphase chromosomes with one genomic clone as a probe, the human glutaredoxin gene was localized to chromosomal region 5q14. This localization at chromosome 5 was in agreement with the somatic cell hybrid analysis, using DNA from a human-hamster and a human-mouse hybrid panel and using a human glutaredoxin cDNA as a probe. 13 refs., 2 figs.

• OSTI ID: 459013
• Journal Information: Genomics, Vol. 32, Issue 3; Other Information: PBD: 5 Mar 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
HUMAN CHROMOSOME 5
GENETIC MAPPING
CHROMOSOMAL ABERRATIONS
PROTEINS
DNA-CLONING
DNA SEQUENCING
STRUCTURE-ACTIVITY RELATIONSHIPS
NEOPLASMS
GENETICS
SOMATIC CELLS
HYBRIDIZATION
GLUTATHIONE
DNA HYBRIDIZATION
FLUORESCENCE
PROBES
OXIDOREDUCTASES
HEREDITARY DISEASES
CONGENITAL MALFORMATIONS