An integrated map with cosmid/PAC contigs of a 4-Mb down syndrome critical region

Journal Article · Tue Mar 05 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0132· OSTI ID:459004

Osoegawa, Kazutoyo; Susukida, Rie; Okano, Saishi ^[1]

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1. Inst. of Physical and Chemical Research, Tsukuba (Japan); and others

The major phenotypic features of Down syndrome have been correlated with partial trisomies of chromosome 21, allowing us to define the candidate gene region to a 4-Mb segment on the 21q22.2 band. We present here a high resolution physical map with megabase-sized cosmid/PAC contigs. This ordered clone library has provided unique material for the integration of a variety of mappable objects, including exons, cDNAs, restriction sites etc. Furthermore, our results have emplified a strategy for the completion of the chromosome 21 map to sequencing. 42 refs., 5 figs.

• OSTI ID: 459004
• Journal Information: Genomics, Vol. 32, Issue 3; Other Information: PBD: 5 Mar 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
HUMAN CHROMOSOME 21
GENETIC MAPPING
DNA-CLONING
DNA SEQUENCING
CHROMOSOMAL ABERRATIONS
RESOLUTION
COSMIDS
DESIGN
CONTIGS
DOWNS SYNDROME
GENETICS
GENES
TRANSCRIPTION
MENTAL DISORDERS
POLYMERASE CHAIN REACTION
DNA HYBRIDIZATION
HEREDITARY DISEASES
BIOLOGICAL MARKERS
PHENOTYPE