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Title: Stimulation of indigenous microbes to bioremediate oil-contaminated soils

Abstract

The purpose of our study was to determine the effectiveness of bioremediation of two-oil-contaminated soils by stimulating indigeneous oil-degrading microbes. The soil treatment consisted of tilling, liming, and fertilizing, and bioremediation was monitored by changes in the microbial populations (MPs) and by changes in total petroleum hydrocarbons (TPHs) in the soils. Location one was a 12 {times} 12 ft plot that had been repeatedly contaminated with pressure pump oil for 8 years. After treatment, the MP increased 400-fold in 3 weeks and remained unchanged for 6 more weeks. The initial TPH was 18,500 ppm, and it declined steadily to 3,000 ppm by 7 weeks. Location two was a rectangular plot approximately 20 {times} 500 ft on a hillside where about 50 bbl of crude oil had been spilled. MPs and TPHs were made at the three sites: site 1, top of hill where spill occurred; site 2, midway uphill; and site 3, about 22 ft below site 1. The initial MPs at the three sites increased a thousandfold in 10 weeks after treatment. Initial TPHs at the three sites ranged from 15,000 to 20,000 ppm and remained unchanged for 26 weeks. At week 30, the TPHs of sites 1 andmore » 2 were less than 10,000 ppm; site 3 required an extra treatment and 12 more weeks before the TPH was below 10,000 ppm. At locations one and two, the decreases in TPH were inversely proportional to the increases in MPs. Bioremediation by stimulating indigenous microbes was effective and, in terms of materials and labor, efficient.« less

Authors:
; ; ;  [1];  [2]
  1. Univ. of Texas, Tyler, TX (United States)
  2. MAP Productions, Inc., Tyler, TX (United States)
Publication Date:
OSTI Identifier:
45238
Report Number(s):
CONF-941065-
TRN: 95:001437-0061
Resource Type:
Conference
Resource Relation:
Conference: 44. annual convention of the Gulf Coast Association of Geological Societies and the 41st annual convention of the Gulf Coast Section of the Society of Economic Paleontologists and Mineralogists, Austin, TX (United States), 5-7 Oct 1994; Other Information: PBD: 1994; Related Information: Is Part Of Gulf Coast Association of Geological Societies: Transactions. Volume 44; Major, R.P. [ed.]; PB: 847 p.
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; SOILS; CONTAMINATION; OIL SPILLS; REMEDIAL ACTION; MICROORGANISMS; STIMULATION

Citation Formats

Stewart, J R, Williams, D L, Kriel, K D, Stewart, G A, and Hulse, R C. Stimulation of indigenous microbes to bioremediate oil-contaminated soils. United States: N. p., 1994. Web.
Stewart, J R, Williams, D L, Kriel, K D, Stewart, G A, & Hulse, R C. Stimulation of indigenous microbes to bioremediate oil-contaminated soils. United States.
Stewart, J R, Williams, D L, Kriel, K D, Stewart, G A, and Hulse, R C. 1994. "Stimulation of indigenous microbes to bioremediate oil-contaminated soils". United States.
@article{osti_45238,
title = {Stimulation of indigenous microbes to bioremediate oil-contaminated soils},
author = {Stewart, J R and Williams, D L and Kriel, K D and Stewart, G A and Hulse, R C},
abstractNote = {The purpose of our study was to determine the effectiveness of bioremediation of two-oil-contaminated soils by stimulating indigeneous oil-degrading microbes. The soil treatment consisted of tilling, liming, and fertilizing, and bioremediation was monitored by changes in the microbial populations (MPs) and by changes in total petroleum hydrocarbons (TPHs) in the soils. Location one was a 12 {times} 12 ft plot that had been repeatedly contaminated with pressure pump oil for 8 years. After treatment, the MP increased 400-fold in 3 weeks and remained unchanged for 6 more weeks. The initial TPH was 18,500 ppm, and it declined steadily to 3,000 ppm by 7 weeks. Location two was a rectangular plot approximately 20 {times} 500 ft on a hillside where about 50 bbl of crude oil had been spilled. MPs and TPHs were made at the three sites: site 1, top of hill where spill occurred; site 2, midway uphill; and site 3, about 22 ft below site 1. The initial MPs at the three sites increased a thousandfold in 10 weeks after treatment. Initial TPHs at the three sites ranged from 15,000 to 20,000 ppm and remained unchanged for 26 weeks. At week 30, the TPHs of sites 1 and 2 were less than 10,000 ppm; site 3 required an extra treatment and 12 more weeks before the TPH was below 10,000 ppm. At locations one and two, the decreases in TPH were inversely proportional to the increases in MPs. Bioremediation by stimulating indigenous microbes was effective and, in terms of materials and labor, efficient.},
doi = {},
url = {https://www.osti.gov/biblio/45238}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}

Conference:
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