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Title: Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide pyrophosphatase gene (PDNP2)

Abstract

Phosphodiesterase I/nucleotide pyrophosphatase is a widely expressed membrane-bound enzyme that cleaves diester bonds of a variety of substrates. We have cloned brain-type cDNA for this enzyme from rat brain and designated it PD-I{alpha}. In this study we have isolated cDNA and genomic DNA encoding human PD-I{alpha}. Human PD-I{alpha} cDNA, designated PDNP2 in HGMW nomenclature, has a 2589-nucleotide open reading frame encoding a polypeptide of 863 amino acids with a calculated M{sub r} of 99,034. Northern blot analysis revealed that human PD-I{alpha} transcript was present in brain, lung, placenta, and kidney. The database analysis showed that human PD-I{alpha} was identical with human autotaxin (ATX), a novel tumor motility-stimulating factor, except that human PD-I{alpha} lacks 156 nucleotides and 52 amino acids of human ATX. Human PD-I{alpha} and human ATX are likely to be alternative splicing products from the same gene. The 5{prime} region of the human PDNP2 gene contains four putative binding sites of transcription factor Sp1 without typical TATA or CAAT boxes, and there is a potential octamer binding motif in intron 2. From the results of fluorescence in situ hybridization, the human PDNP2 gene is located at chromosome 8q24.1. 17 refs., 3 figs.

Authors:
; ;  [1]
  1. Kobe Univ. School of Medicine (Japan); and others
Publication Date:
OSTI Identifier:
446989
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 30; Journal Issue: 2; Other Information: PBD: 20 Nov 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOME 8; GENETIC MAPPING; PHOSPHODIESTERASES; SPLICING; DNA-CLONING; TRANSCRIPTION; STRUCTURE-ACTIVITY RELATIONSHIPS; ENZYME ACTIVITY; PYROPHOSPHATES; AMINO ACIDS; DNA HYBRIDIZATION; FLUORESCENCE; NUCLEOTIDES; INTRONS; DNA SEQUENCING

Citation Formats

Kawagoe, Hiroyuki, Soma, Osamu, and Goji, Junko. Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide pyrophosphatase gene (PDNP2). United States: N. p., 1995. Web. doi:10.1006/geno.1995.0036.
Kawagoe, Hiroyuki, Soma, Osamu, & Goji, Junko. Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide pyrophosphatase gene (PDNP2). United States. https://doi.org/10.1006/geno.1995.0036
Kawagoe, Hiroyuki, Soma, Osamu, and Goji, Junko. 1995. "Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide pyrophosphatase gene (PDNP2)". United States. https://doi.org/10.1006/geno.1995.0036.
@article{osti_446989,
title = {Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide pyrophosphatase gene (PDNP2)},
author = {Kawagoe, Hiroyuki and Soma, Osamu and Goji, Junko},
abstractNote = {Phosphodiesterase I/nucleotide pyrophosphatase is a widely expressed membrane-bound enzyme that cleaves diester bonds of a variety of substrates. We have cloned brain-type cDNA for this enzyme from rat brain and designated it PD-I{alpha}. In this study we have isolated cDNA and genomic DNA encoding human PD-I{alpha}. Human PD-I{alpha} cDNA, designated PDNP2 in HGMW nomenclature, has a 2589-nucleotide open reading frame encoding a polypeptide of 863 amino acids with a calculated M{sub r} of 99,034. Northern blot analysis revealed that human PD-I{alpha} transcript was present in brain, lung, placenta, and kidney. The database analysis showed that human PD-I{alpha} was identical with human autotaxin (ATX), a novel tumor motility-stimulating factor, except that human PD-I{alpha} lacks 156 nucleotides and 52 amino acids of human ATX. Human PD-I{alpha} and human ATX are likely to be alternative splicing products from the same gene. The 5{prime} region of the human PDNP2 gene contains four putative binding sites of transcription factor Sp1 without typical TATA or CAAT boxes, and there is a potential octamer binding motif in intron 2. From the results of fluorescence in situ hybridization, the human PDNP2 gene is located at chromosome 8q24.1. 17 refs., 3 figs.},
doi = {10.1006/geno.1995.0036},
url = {https://www.osti.gov/biblio/446989}, journal = {Genomics},
number = 2,
volume = 30,
place = {United States},
year = {Mon Nov 20 00:00:00 EST 1995},
month = {Mon Nov 20 00:00:00 EST 1995}
}