Determination and regional assignment of grouped sets of microclones in chromosome 1pter-p35
- Research Inst. of Molecular Pathology, Vienna (Austria)
In an approach to mapping physically the most distal 30 Mb of human chromosome 1p, region-specific clone libraries were generated by microdissection and microcloning, PFGE blot hybridization of single or low-copy microclones against rare-cutter digests of genomic DNA revealed physical linkage for groups of markers. Supplementary PFGE analysis of 31 1p36-p35-specific probes for genetically mapped loci established a total of 15 grouped sets, consisting of altogether 69 markers. Twelve of the grouped sets were located in 1pter-p36.12, as revealed by microcell hybrid mapping; the remaining three were localized proximal to 1p36.12. Regional assignment and ordering of most grouped sets was achieved either by evaluating the included genetic markers or by fluorescence in situ hybridization of representative probes. The genomic extent of individual grouped sets encompassed between 1100 and 2100 kb, covering a total of approximately 22 Mb of the distal chromosome 1p region. One particular grouped set was shown to contain seven polymorphic marker loci that were previously suggested to be distributed across the entire 1pter-p35 region. The increase in the number of hybridization marker probes in 1p36 and their physical mapping is expected to facilitate positional cloning experiments in this region; in particular, the construction of clone contigs may be greatly facilitated. 44 refs., 3 figs., 3 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 443860
- Journal Information:
- Genomics, Vol. 29, Issue 3; Other Information: PBD: 10 Oct 1995
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
99 MATHEMATICS
COMPUTERS
INFORMATION SCIENCE
MANAGEMENT
LAW
MISCELLANEOUS
DNA-CLONING
DATA BASE MANAGEMENT
HUMAN CHROMOSOME 1
GENETIC MAPPING
CHROMOSOMAL ABERRATIONS
MUTATION FREQUENCY
CONTIGS
DESIGN
GENES
GENE MUTATIONS
DETECTION
ELECTROPHORESIS
DNA HYBRIDIZATION
BIOLOGICAL MARKERS
PROBES
FLUORESCENCE
HEREDITARY DISEASES
GENOTYPE