Isolation and characterization of the human MRE11 homologue
- Harvard Medical School, Boston, MA (United States); and others
Mutation of the Saccharomyces cerevisiae RAD52 epistasis group gene, MRE11, blocks meiotic recombination, confers profound sensitivity to double-strand break damage, and has a hyperrecombinational phenotype in mitotic cells. We isolated a highly conserved human MRE11 homologue using a two-hybrid screen for DNA ligase I-interacting proteins. Human MRE11 shares approximately 50% identity with its yeast counterpart over the N-terminal half of the protein. MRE11 is expressed at the highest levels in proliferating tissues, but is also observed in other tissues. The MRE11 locus maps to human chromosome 11q21 in a region frequently associated with cancer-related chromosomal abnormalities. A MRE11-related locus was found on chromosome 7q11.2-q11.3. 60 refs., 4 figs.
- OSTI ID:
- 435020
- Journal Information:
- Genomics, Vol. 29, Issue 1; Other Information: PBD: 1 Sep 1995
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
GENES
GENETIC MAPPING
GENE RECOMBINATION
GENE REGULATION
SOMATIC MUTATIONS
DNA SEQUENCING
HUMAN CHROMOSOMES
STRAND BREAKS
CHROMOSOMAL ABERRATIONS
HUMAN CHROMOSOME 7
DNA REPAIR
ANIMAL CELLS
ONCOGENIC TRANSFORMATIONS
CELL DIVISION
SACCHAROMYCES CEREVISIAE
MEIOSIS
PROTEINS
DNA HYBRIDIZATION