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Title: Phytoremediation of TNT: C. roseus hairy roots as a model system

Abstract

Widespread contamination by 2,4,6-trinitrotoluene (TNT) of Soil exists at former munitions production and handling facilities. Phytoremediation may be an effective alternative to existing methods of TNT remediation: incineration is highly expensive and recalcitrant reduction products are formed in composting. Recently, the intrinsic ability of plants to transform TNT has been demonstrated using hairy root cultures of Catharanthus roseus as a model system. Kinetic studies were performed at concentrations of 30 and 50 mg/L TNT in growth medium. The pseudo-first order rate constants for disappearance ranged from 0.0103 to 0.0161 (L/g-day); TNT disappears completely within seven to ten days of exposure. The fate of the TNT molecule in plants is also currently under study, mass balance studies were performed with 1-{sup 14}C TNT. After a seven day exposure period, 72% of the label was associated with the roots and 30% was associated with the medium. However, HPLC analysis shows that less than 5% (wt%) of the TNT added is recoverable from both the plants and the media in the form of reduction products. 11 refs., 2 figs.

Authors:
; ;  [1]
  1. Rice Univ., Houston, TX (United States)
Publication Date:
OSTI Identifier:
430595
Report Number(s):
CONF-960730-
TRN: 96:006487-0106
Resource Type:
Conference
Resource Relation:
Conference: 5. world congress of chemical engineering, San Diego, CA (United States), 14-18 Jul 1996; Other Information: PBD: 1996; Related Information: Is Part Of The 5th World Congress of chemical engineering: Technologies critical to a changing world. Volume III: Emerging energy technologies clean technologies remediation and emission control fuels and petrochemicals; PB: 1118 p.
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; TNT; BIOCONVERSION; BIODEGRADATION; BIOADSORBENTS; BIOCHEMICAL REACTION KINETICS; ROOTS

Citation Formats

Lauritzen, J R, Hughes, J B, and Shanks, J V. Phytoremediation of TNT: C. roseus hairy roots as a model system. United States: N. p., 1996. Web.
Lauritzen, J R, Hughes, J B, & Shanks, J V. Phytoremediation of TNT: C. roseus hairy roots as a model system. United States.
Lauritzen, J R, Hughes, J B, and Shanks, J V. 1996. "Phytoremediation of TNT: C. roseus hairy roots as a model system". United States.
@article{osti_430595,
title = {Phytoremediation of TNT: C. roseus hairy roots as a model system},
author = {Lauritzen, J R and Hughes, J B and Shanks, J V},
abstractNote = {Widespread contamination by 2,4,6-trinitrotoluene (TNT) of Soil exists at former munitions production and handling facilities. Phytoremediation may be an effective alternative to existing methods of TNT remediation: incineration is highly expensive and recalcitrant reduction products are formed in composting. Recently, the intrinsic ability of plants to transform TNT has been demonstrated using hairy root cultures of Catharanthus roseus as a model system. Kinetic studies were performed at concentrations of 30 and 50 mg/L TNT in growth medium. The pseudo-first order rate constants for disappearance ranged from 0.0103 to 0.0161 (L/g-day); TNT disappears completely within seven to ten days of exposure. The fate of the TNT molecule in plants is also currently under study, mass balance studies were performed with 1-{sup 14}C TNT. After a seven day exposure period, 72% of the label was associated with the roots and 30% was associated with the medium. However, HPLC analysis shows that less than 5% (wt%) of the TNT added is recoverable from both the plants and the media in the form of reduction products. 11 refs., 2 figs.},
doi = {},
url = {https://www.osti.gov/biblio/430595}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 31 00:00:00 EST 1996},
month = {Tue Dec 31 00:00:00 EST 1996}
}

Conference:
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