Mechanistic studies of partial-filing micellar electrokinetic chromatography

Journal Article · Sun Sep 15 00:00:00 EDT 1996 · Analytical Chemistry (Washington)

DOI:https://doi.org/10.1021/ac951137o· OSTI ID:420883

Nelson, W M; Lee, C S ^[1]

---

1. Ames National Lab., IA (United States)

The need for coupling micellar electrokinetic chromatography (MEKC) with electrospray mass spectrometry initiates the development of partial-filling MEKC. In comparison with conventional MEKC, only a small portion of the capillary is filled with a micellar solution for performing the separation in partial-filling MEKC. Analytes first migrate into the micellar plug, where the separation occurs, and then into the leading electrophoresis buffer, which is free of surfactants. A theoretical model is proposed for predicting the separation behavior of triazine herbicides in partial-filling MEKC. The comparisons between conventional and partial-filling MEKC in terms of separation efficiency and resolution of triazine herbicides are presented and discussed. The optimization techniques, possible applications, and advantages of partial-filling MEKC are similarly addressed. 11 refs., 6 figs., 5 tabs.

• OSTI ID: 420883
• Journal Information: Analytical Chemistry (Washington), Vol. 68, Issue 18; Other Information: PBD: 15 Sep 1996
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
HERBICIDES
CHEMICAL ANALYSIS
TRIAZINES
SEPARATION PROCESSES
MICELLAR SYSTEMS
CHROMATOGRAPHY
ELECTRODYNAMICS
MATHEMATICAL MODELS
ELECTROPHORESIS