Expression of heat shock gene construct in transformed fish cell culture (RTG-2) after toxicant exposure
- Univ. of Maryland, Baltimore, MD (United States). Center for Marine Biotechnology
- Univ. of Calgary, Alberta (Canada). Dept. of Biological Sciences
- Univ. of Connecticut, Storrs, CT (United States)
The authors have developed a transformed fish cell line (RTG-2) that can be used as a noninvasive compliment for the evaluation of toxicants (metals and organometal compounds) in the laboratory. Gene transfer and integration into the cell line was accomplished through the insertion of multiple copies of a heat shock gene that had been fused to the structural gene for firefly luciferase (luc). Transcription of the hsp70-luc transgene was inducible through heat-shock and acute exposure to metals (Cd, Cu) and organometal (TBT) compounds in the laboratory. Induction resulted in a rapid luminescence intensity proportional to the concentration of luciferase activity and presents a novel noninvasive diagnostic tool that produced dose-response curves comparable to conventional trout acute studies.
- OSTI ID:
- 398173
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9649%%381
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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