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Title: Production of acetone-butanol-ethanol from corn mash and molasses in batch fermentation

Abstract

The production of solvents from corn mash and molasses in batch fermentation using Clostridium acetobutylicum P 262 was examined. The content of saccharose of beet molasses used in experiments is determined by using the gravimetric method (52.45% saccharose). The quantities of molasses that are used in the nutrient medium are calculated after doing the above determination. The samples of fermentation liquid are taken within a certain time, the determination of saccharose is done by using the same method, and all the saccharose is converted by the microorganism to organic end products. The quantitative and qualitative determination of acetone-butanol has been made by using gas chromatography. On the other hand, using the three isolation way, three different cultures are obtained, and with microscopic observations, the cultures obtained are of the C. acetobutylicum genus. According to the literature values, the concentration of maximum mixed solvent formed during fermentation is about 2%. This is seen in this experiment. There is only a slight difference from this value. This difference is caused by another organic product that is formed during fermentation. 9 refs., 1 fig., 1 tab.

Authors:
;  [1]
  1. Istanbul Technical Univ. (Turkey)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
393887
Resource Type:
Journal Article
Journal Name:
Applied Biochemistry and Biotechnology
Additional Journal Information:
Journal Volume: 56; Journal Issue: 2; Other Information: PBD: Feb 1996
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; MAIZE; BATCH CULTURE; FERMENTATION; MOLASSES; MIXED SOLVENTS; PRODUCTION; ACETONE; BUTANOLS; ETHANOL; BIOMASS; CLOSTRIDIUM ACETOBUTYLICUM; GAS CHROMATOGRAPHY; SACCHAROSE

Citation Formats

Guevenilir, Y A, and Deveci, N. Production of acetone-butanol-ethanol from corn mash and molasses in batch fermentation. United States: N. p., 1996. Web. doi:10.1007/BF02786948.
Guevenilir, Y A, & Deveci, N. Production of acetone-butanol-ethanol from corn mash and molasses in batch fermentation. United States. https://doi.org/10.1007/BF02786948
Guevenilir, Y A, and Deveci, N. 1996. "Production of acetone-butanol-ethanol from corn mash and molasses in batch fermentation". United States. https://doi.org/10.1007/BF02786948.
@article{osti_393887,
title = {Production of acetone-butanol-ethanol from corn mash and molasses in batch fermentation},
author = {Guevenilir, Y A and Deveci, N},
abstractNote = {The production of solvents from corn mash and molasses in batch fermentation using Clostridium acetobutylicum P 262 was examined. The content of saccharose of beet molasses used in experiments is determined by using the gravimetric method (52.45% saccharose). The quantities of molasses that are used in the nutrient medium are calculated after doing the above determination. The samples of fermentation liquid are taken within a certain time, the determination of saccharose is done by using the same method, and all the saccharose is converted by the microorganism to organic end products. The quantitative and qualitative determination of acetone-butanol has been made by using gas chromatography. On the other hand, using the three isolation way, three different cultures are obtained, and with microscopic observations, the cultures obtained are of the C. acetobutylicum genus. According to the literature values, the concentration of maximum mixed solvent formed during fermentation is about 2%. This is seen in this experiment. There is only a slight difference from this value. This difference is caused by another organic product that is formed during fermentation. 9 refs., 1 fig., 1 tab.},
doi = {10.1007/BF02786948},
url = {https://www.osti.gov/biblio/393887}, journal = {Applied Biochemistry and Biotechnology},
number = 2,
volume = 56,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 1996},
month = {Thu Feb 01 00:00:00 EST 1996}
}