Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to embryos of the fathead minnow (Pimephales promelas, Rafinesque)

Olivieri, C; Cooper, K

Title: Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to embryos of the fathead minnow (Pimephales promelas, Rafinesque)

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Abstract

This study correlates the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) residue and its toxicity to the fathead minnow embryo exposed to levels lower than the TCDD solubility range. The TCDD tissue doses in the embryo/larvae were related to gross lesions, delayed hatching, and death. A 7-day bioconcentration factor on a lipid basis (BCF{sub L}) was calculated. Embryos at the blatsula stage were exposed to [{sup 3}H] TCDD water concentrations (0.37, 0.59, 1.2, 2.83, and 10.1 6 ng/L), a nontreatment control and a solvent carrier control. Lesions were qualified as mild and severe based on their onset and their pathogenesis. Thus, mild lesions developed just prior to hatching or post-hatching at the fifth day of development. The severe lesions appeared at the third day of development. Embryos with tissue doses {le}2.46 {+-} 1.34 ng/g consistently developed mild lesions, while embryos with tissue doses {ge} 12.07 {+-} 4.91 ng/g developed severe lesions. The lowest observable adverse effect level was 0.04 {+-} 0.02 ng/g. The calculated 7-day ED{sub 50} was 0.14 ng/g. There was no delayed hatching at any of the concentrations tested. Embryo death occurred at a tissue concentration {ge}2.46 ng/g. The calculated 7-day LD{sub 50} was 25.71 ng/g. The relationship between the concentration of TCDDmore »« less

Authors:

Olivieri, C; Cooper, K ^[1]

Rutgers Univ., New Brunswick, NJ (United States). Cook Coll.

Publication Date:: Sun Dec 31 00:00:00 EST 1995

OSTI Identifier:: 367546

Report Number(s):: CONF-9511137-
ISBN 1-880611-03-1; TRN: IM9640%%292

Resource Type:: Conference

Resource Relation:: Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; DIOXIN; TOXICITY; WATER POLLUTION; BIOLOGICAL INDICATORS; FATHEAD MINNOW; BIOLOGICAL STRESS; ANIMAL TISSUES; PATHOLOGICAL CHANGES; DOSE-RESPONSE RELATIONSHIPS; AGE DEPENDENCE

Citation Formats


                    Olivieri, C, and Cooper, K. Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to embryos of the fathead minnow (Pimephales promelas, Rafinesque).  United States: N. p., 1995. 
        Web.

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                    Olivieri, C, & Cooper, K. Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to embryos of the fathead minnow (Pimephales promelas, Rafinesque).  United States.

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                    Olivieri, C, and Cooper, K. 1995.  
        "Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to embryos of the fathead minnow (Pimephales promelas, Rafinesque)".  United States.

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@article{osti_367546,

  title        = {Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to embryos of the fathead minnow (Pimephales promelas, Rafinesque)},

  author       = {Olivieri, C and Cooper, K},

  abstractNote = {This study correlates the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) residue and its toxicity to the fathead minnow embryo exposed to levels lower than the TCDD solubility range. The TCDD tissue doses in the embryo/larvae were related to gross lesions, delayed hatching, and death. A 7-day bioconcentration factor on a lipid basis (BCF{sub L}) was calculated. Embryos at the blatsula stage were exposed to [{sup 3}H] TCDD water concentrations (0.37, 0.59, 1.2, 2.83, and 10.1 6 ng/L), a nontreatment control and a solvent carrier control. Lesions were qualified as mild and severe based on their onset and their pathogenesis. Thus, mild lesions developed just prior to hatching or post-hatching at the fifth day of development. The severe lesions appeared at the third day of development. Embryos with tissue doses {le}2.46 {+-} 1.34 ng/g consistently developed mild lesions, while embryos with tissue doses {ge} 12.07 {+-} 4.91 ng/g developed severe lesions. The lowest observable adverse effect level was 0.04 {+-} 0.02 ng/g. The calculated 7-day ED{sub 50} was 0.14 ng/g. There was no delayed hatching at any of the concentrations tested. Embryo death occurred at a tissue concentration {ge}2.46 ng/g. The calculated 7-day LD{sub 50} was 25.71 ng/g. The relationship between the concentration of TCDD in the water (x = ng/L) and in the tissue dose (y = ng/g) fitted a linear curve y = {minus}1,143 + 3,792.5x, r{sup 2} = 0.98. The calculated BCF{sub L} (wet weight) was dose dependent. Embryos with tissue doses of 0.04 and 0.16 ng/g had BCF{sub L} of 2,700 and 3,325, respectively. Embryos with tissue doses of 2.46, 12.07, and 37.07 ng/g, had a BCF{sub L} of 104,225, 106,625 and 91,075, respectively.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/367546},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Dec 31 00:00:00 EST 1995},

  month        = {Sun Dec 31 00:00:00 EST 1995}

}

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