Micro-scale analysis for the determination PAHs in benthic macroinvertebrates
- Environmental Protection Agency, Duluth, MN (United States)
- Integrated Lab. Systems, Duluth, MN (United States)
- Univ. of Wisconsin, Superior, WI (United States). Lake Superior Research Inst.
The amount of tissue required for analysis of trace concentrations of non-ionic organic chemicals can be fairly large, e.g., as high as 20 g. However, in order to routinely analyze residues in smaller sediment-dwelling organisms, modified analytical residue techniques are required to accommodate very small sample sizes (e.g., 0.05 to 0.2 g) to achieve desired detection limits. The objective of this study was to optimize analytical techniques for the measurement of polycyclic aromatic hydrocarbon (PAH) residues in two species of freshwater benthic macroinvertebrates, Lumbriculus variegatus and Chironomus tentans. There are several ways of increasing sensitivity for tissue residue analysis, including altering cleanup steps, reducing compound loss, and optimizing instrument detection limits. These techniques were applied to tissue samples from several PAH toxicity and bioaccumulation studies with L. variegatus and C. tentans larvae. Tissues were homogenized and extracted using a micro-homogenizer, and the extracts were analyzed using high pressure liquid chromatography (HPLC) with fluorescence detection. The final method, which will be described in this presentation, provides reproducible data that exceed typical quality assurance requirements and results in detection limits as low as 10 ng/g in sample sizes as small as 0.05 g.
- OSTI ID:
- 367459
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9640%%205
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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