Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption: A Plume Sharpening Effect

Journal Article · Thu Jul 01 00:00:00 EDT 1999 · Physical Review Letters

DOI:https://doi.org/10.1103/PhysRevLett.83.444· OSTI ID:355463

Puretzky, A A; Geohegan, D B; Hurst, G B; Buchanan, M V ^[1]; Luk`yanchuk, B S ^[2]

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1. Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 (United States)
2. General Physics Institute, Russian Academy of Sciences, 117942 Moscow (Russia)

The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNasethinspthinspI proteins shows that these heavy molecules (30thinsp000thinspthinspDa) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139thinspthinspDa). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components. {copyright} {ital 1999} {ital The American Physical Society}

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• DOE Contract Number: AC05-96OR22464
• OSTI ID: 355463
• Journal Information: Physical Review Letters, Vol. 83, Issue 2; Other Information: PBD: Jul 1999
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
BIOLOGICAL MATERIALS
PROTEINS
PLUMES
DESORPTION
LASER RADIATION
IMAGES
IONIZATION
FLUORESCENCE
ANGULAR DISTRIBUTION
MOLECULES
VAPORS