Identification of cytochrome P4501A inducers in complex mixtures of polycyclic aromatic hydrocarbons
- Michigan State Univ., East Lansing, MI (United States)
- Univ. of Wisconsin, Stevens Point, WI (United States). Coll. of Natural Resources
An in vitro ethoxyresorufin O-deethylase (EROD) assay was used to study the ability of individual polycyclic aromatic hydrocarbons (PAHs) and mixtures of PAHs to induce Ah receptor (AhR) mediated cytochrome P4501A activity in PLHC-1 fish hepatoma cells. The purpose was to identify the most potent inducers from a set of thirteen separate PAHs and describe interactions occurring in complex mixtures of these PAHs. Where possible, potency was expressed in terms of 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TCDD-EQ) by normalizing the PAH results to a TCDD standard curve. The most potent inducers were benzo(k)fluoranthene > benzo(a)pyrene {approx} benzo(b)fluoranthene > chrysene {approx} benzo(a)anthracene. At equal concentrations, these PAHs yielded potencies of 1670, 940, 655, 255, and 185 pg TCDD-EQ/g, respectively. Analysis of various mixtures of the thirteen PAHs suggested that complex interactions may be occurring.
- OSTI ID:
- 320945
- Report Number(s):
- CONF-9704249-; TRN: IM9910%%135
- Resource Relation:
- Conference: 7. symposium on toxicology and risk assessment: ultraviolet radiation and the environment, St. Louis, MO (United States), 7-9 Apr 1997; Other Information: PBD: 1998; Related Information: Is Part Of Environmental toxicology and risk assessment: Seventh volume; Little, E.E.; DeLonay, A.J. [eds.] [Geological Survey, Columbia, MO (United States)]; Greenberg, B.M. [ed.] [Univ. of Waterloo, Ontario (Canada). Dept. of Biology]; PB: 427 p.; ASTM special technical publication, Volume 1333
- Country of Publication:
- United States
- Language:
- English
Similar Records
Glucocorticoid-mediated potentiation of cytochrome P4501A induction in a fish hepatoma cell line
Assessment of toxic potency of complex mixtures of PAHs from Lincoln Creek, Milwaukee, WI