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Title: Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)

Abstract

There is increasing evidence that neurotrophins and their receptors play an important role in regulating development of both the central and the peripheral nervous systems. Human TRK-A (NTRK1) and TRK-C (NTRK3) have been cloned and sequenced, but only a truncated form of human TRK-B has been published. Therefore, we isolated complementary DNAs spanning the entire coding region of both human full-length and truncated forms of TRK-B from human brain cDNA libraries. Human full-length TRK-B codes for a protein of 822 amino acid residues. The putative mature peptide sequence is 49% homologous to human TRK-A and 55% full-length human TRK-C, with 40% amino acid identity among TRK-A, -B, and -C. Nine of 13 cysteine residues, 4 of 12 N-glycosylation sites in the extracellular domain, and 10 of 13 tyrosine residues in the intracellular domain are conserved among human TRK-A, -B, and -C. There is a cluster of 10 serine residues in the juxtamembrane region of TRK-B that is absent in TRK-A. Two major sizes of TRK-B transcripts were expressed in human brain. Northern blot analysis using probes specific for the extracellular or the tyrosine kinase domain revealed that the 9.5-kb band encodes the truncated from of TRK-B mRNA. By fluorescence inmore » situ hybridization and somatic cell hybrid mapping, the human TRK-B gene was localized to chromosome 9q22.1. 31 refs., 4 figs.« less

Authors:
; ;  [1]
  1. Children`s Hospital of Philadelphia, PA (United States); and others
Publication Date:
OSTI Identifier:
250167
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 25; Journal Issue: 2; Other Information: PBD: 20 Jan 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; DNA-CLONING; DNA SEQUENCING; TRANSCRIPTION; GENETIC MAPPING; GENE REGULATION; HUMAN CHROMOSOME 9; RECEPTORS; TYROSINE; PHOSPHOTRANSFERASES; DNA HYBRIDIZATION; PEPTIDES; FLUORESCENCE; SOMATIC CELLS; AMINO ACID SEQUENCE; POLYMERASE CHAIN REACTION

Citation Formats

Nakagawara, A, Liu, X G, and Ikegaki, N. Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2). United States: N. p., 1995. Web. doi:10.1016/0888-7543(95)80055-Q.
Nakagawara, A, Liu, X G, & Ikegaki, N. Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2). United States. https://doi.org/10.1016/0888-7543(95)80055-Q
Nakagawara, A, Liu, X G, and Ikegaki, N. 1995. "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)". United States. https://doi.org/10.1016/0888-7543(95)80055-Q.
@article{osti_250167,
title = {Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)},
author = {Nakagawara, A and Liu, X G and Ikegaki, N},
abstractNote = {There is increasing evidence that neurotrophins and their receptors play an important role in regulating development of both the central and the peripheral nervous systems. Human TRK-A (NTRK1) and TRK-C (NTRK3) have been cloned and sequenced, but only a truncated form of human TRK-B has been published. Therefore, we isolated complementary DNAs spanning the entire coding region of both human full-length and truncated forms of TRK-B from human brain cDNA libraries. Human full-length TRK-B codes for a protein of 822 amino acid residues. The putative mature peptide sequence is 49% homologous to human TRK-A and 55% full-length human TRK-C, with 40% amino acid identity among TRK-A, -B, and -C. Nine of 13 cysteine residues, 4 of 12 N-glycosylation sites in the extracellular domain, and 10 of 13 tyrosine residues in the intracellular domain are conserved among human TRK-A, -B, and -C. There is a cluster of 10 serine residues in the juxtamembrane region of TRK-B that is absent in TRK-A. Two major sizes of TRK-B transcripts were expressed in human brain. Northern blot analysis using probes specific for the extracellular or the tyrosine kinase domain revealed that the 9.5-kb band encodes the truncated from of TRK-B mRNA. By fluorescence in situ hybridization and somatic cell hybrid mapping, the human TRK-B gene was localized to chromosome 9q22.1. 31 refs., 4 figs.},
doi = {10.1016/0888-7543(95)80055-Q},
url = {https://www.osti.gov/biblio/250167}, journal = {Genomics},
number = 2,
volume = 25,
place = {United States},
year = {Fri Jan 20 00:00:00 EST 1995},
month = {Fri Jan 20 00:00:00 EST 1995}
}