Rapid mapping of NotI linking clones with differential hybridization and Alu-PCR
- Karolinska Institute, Stockholm (Sweden)
- Karolinska Institute, Stockholm (Sweden); and others
For construction of a NotI restriction map of the humane genome, the isolation and mapping of unique NotI linking clones represent important and critical steps. Recently the authors have shown that an Alu-PCR approach can be used for isolation of NotI linking clones from defined regions of the chromosomes. This represents a useful method for isolating and analyzing a small number of clones, but it would be laborious to use it for mapping many NotI linking clones simultaneously. Here they suggest another modification of Alu-PCR for rapid concurrent mapping of many NotI linking clones. The results clearly demonstrate the utility of this approach. Seventy-one random NotI linking clones were analyzed. Among them, 65 clones (91.5%) were correctly selected and mapped using this approach. With differential hybridization and Alu-PCR, a significant portion of all human NotI linking clones (>30%) can be rapidly mapped to particular chromosomes or to defined regions of these chromosomes. 31 refs., 3 figs.
- OSTI ID:
- 250044
- Journal Information:
- Genomics, Vol. 21, Issue 3; Other Information: PBD: Jun 1994
- Country of Publication:
- United States
- Language:
- English
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