Effect of 3,3{prime},4,4{prime}5 pentachlorobiphenyl (PCB{number_sign}126) on benzo(a)pyrene induced DNA-damage in the fish hepatoma cell line PLHC-1
- Univ. of Utrecht (Netherlands). Research Inst. of Toxicology
- Netherlands Inst. for Sea Research, Texel (Netherlands)
Benzo(a)pyrene (BaP) can be transformed to 7,8 diol-9,10 epoxide BaP which is a major carcinogen. The cytochrome P45OIAI isoenzyme is playing an important role in this bioactivation. The coplanar PCB{number_sign}126 is a strong inducer of CYPIAI in vivo but also in the PLHC-1 fish cell line. This CYPIAI inducing property of PCB{number_sign}L26 could increase the BaP induced DNA-damage. In this study dose-response relationships for PCB{number_sign}126, BaP and their combination are presented, measured either as CYPIAI (EROD) induction or as DNA strand breaks. Strand breaks were measured by means of an alkaline unwinding assay. BaP is readily metabolized in this cell line and induces strand breaks dose-dependently. PCB induces EROD in a dose-dependant way but does not cause DNA strand breaks significantly. PLHC-1 cells were pre-treated with PCB{number_sign}126 (LOEL,ED50 and maximal EROD induction) and then dosed with BaP (LOEL,ED5 and maximal strand break induction). DNA-strand breaks in the pre-treated cells were compared to cells treated with BaP alone. Results of these combined exposures will be presented and discussed.
- OSTI ID:
- 242350
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9626%%178
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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