skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse

Abstract

The ubiquitously expressed upstream stimulatory factor (USF) involved in the transcription of a wide variety of cellular genes is defined as dimers of c-myc-related proteins, composed of a basic helix-loop-helix/leucine zipper region. The USF family consists of different members that split into two groups: MLTF or USF1 and USF2 or FIR. We present here evidence that USF1 and USF2 are distinct closely related genes in human, rat, and mouse. Based on the recent cloning of rat and human new cDNAs, we have isolated genomic clones encompassing the murine USF2 gene, which consists of at least 10 exons spanning a minimum of 10 kb of genomic DNA. Unexpectedly, the organization of USF2 appears very split up by introns (0.08 to over 6 kb in size), compared to the myc gene structure. The entire gene (but the larger intron) and 1.6 kb of the 5{prime} flanking region were sequenced. This 5{prime} flanking region is GC-rich, contains several putative transcription binding sites, and has no apparent TATA box. Gene mapping of murine USF2 and USF1 has been determined by in situ hybridization, indicating the localization of USF2 on chromosome 7 and of USF1 on chromosomes 1 and 11. 35 refs., 4 figs., 1more » tab.« less

Authors:
; ;  [1]
  1. Institut Cochin de Genetique Moleculaire, Paris (France); and others
Publication Date:
OSTI Identifier:
241064
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 25; Journal Issue: 1; Other Information: PBD: 1 Jan 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; CHROMOSOMES; GENETIC MAPPING; GENES; TRANSCRIPTION; STRUCTURE-ACTIVITY RELATIONSHIPS; DNA SEQUENCING; TRANSCRIPTION FACTORS; PROTEINS; DIMERS; EXONS; DNA-CLONING; MICE; INTRONS; DNA HYBRIDIZATION; POLYMERASE CHAIN REACTION

Citation Formats

Henrion, A A, Martinez, A, and Kahn, A. Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse. United States: N. p., 1995. Web. doi:10.1016/0888-7543(95)80107-W.
Henrion, A A, Martinez, A, & Kahn, A. Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse. United States. https://doi.org/10.1016/0888-7543(95)80107-W
Henrion, A A, Martinez, A, and Kahn, A. 1995. "Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse". United States. https://doi.org/10.1016/0888-7543(95)80107-W.
@article{osti_241064,
title = {Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse},
author = {Henrion, A A and Martinez, A and Kahn, A},
abstractNote = {The ubiquitously expressed upstream stimulatory factor (USF) involved in the transcription of a wide variety of cellular genes is defined as dimers of c-myc-related proteins, composed of a basic helix-loop-helix/leucine zipper region. The USF family consists of different members that split into two groups: MLTF or USF1 and USF2 or FIR. We present here evidence that USF1 and USF2 are distinct closely related genes in human, rat, and mouse. Based on the recent cloning of rat and human new cDNAs, we have isolated genomic clones encompassing the murine USF2 gene, which consists of at least 10 exons spanning a minimum of 10 kb of genomic DNA. Unexpectedly, the organization of USF2 appears very split up by introns (0.08 to over 6 kb in size), compared to the myc gene structure. The entire gene (but the larger intron) and 1.6 kb of the 5{prime} flanking region were sequenced. This 5{prime} flanking region is GC-rich, contains several putative transcription binding sites, and has no apparent TATA box. Gene mapping of murine USF2 and USF1 has been determined by in situ hybridization, indicating the localization of USF2 on chromosome 7 and of USF1 on chromosomes 1 and 11. 35 refs., 4 figs., 1 tab.},
doi = {10.1016/0888-7543(95)80107-W},
url = {https://www.osti.gov/biblio/241064}, journal = {Genomics},
number = 1,
volume = 25,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1995},
month = {Sun Jan 01 00:00:00 EST 1995}
}