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Title: A novel cell weighing method based on the minimum immobilization pressure for biological applications

A novel weighing method for cells with spherical and other regular shapes is proposed in this paper. In this method, the relationship between the cell mass and the minimum aspiration pressure to immobilize the cell (referred to as minimum immobilization pressure) is derived for the first time according to static theory. Based on this relationship, a robotic cell weighing process is established using a traditional micro-injection system. Experimental results on porcine oocytes demonstrate that the proposed method is able to weigh cells at an average speed of 16.3 s/cell and with a success rate of more than 90%. The derived cell mass and density are in accordance with those reported in other published results. The experimental results also demonstrated that this method is able to detect less than 1% variation of the porcine oocyte mass quantitatively. It can be conducted by a pair of traditional micropipettes and a commercial pneumatic micro-injection system, and is expected to perform robotic operation on batch cells. At present, the minimum resolution of the proposed method for measuring the cell mass can be 1.25 × 10{sup −15 }kg. Above advantages make it very appropriate for quantifying the amount of the materials injected into or moved out of the cellsmore » in the biological applications, such as nuclear enucleations and embryo microinjections.« less
Authors:
 [1] ;  [2] ;  [1] ;  [3] ; ; ;  [4]
  1. Robotics and Mechatronics Research Laboratory, Department of Mechanical and Aerospace Engineering, Monash University, Clayton 3800 (Australia)
  2. (China)
  3. Biotechnology Lab of Animal Reproduction, Tianjin Animal Sciences, Tianjin 300112 (China)
  4. Institute of Robotics and Automatic Information System, Nankai University, Tianjin 300071 (China)
Publication Date:
OSTI Identifier:
22494649
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Applied Physics; Journal Volume: 118; Journal Issue: 4; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ANIMAL CELLS; DENSITY; EMBRYOS; MASS; MEASURING METHODS; OOCYTES; RESOLUTION; SPHERICAL CONFIGURATION; WEIGHT