Three-dimensional structure of brain tissue at submicrometer resolution

Journal Article · Thu Jan 28 00:00:00 EST 2016 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4937498· OSTI ID:22494375

Saiga, Rino; Mizutani, Ryuta ^[1]; Inomoto, Chie; Takekoshi, Susumu; Nakamura, Naoya; Tsuboi, Akio; Osawa, Motoki ^[2]; Arai, Makoto; Oshima, Kenichi; Itokawa, Masanari ^[3]; Uesugi, Kentaro; Takeuchi, Akihisa; Terada, Yasuko; Suzuki, Yoshio ^[4]

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1. Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa 259-1292 (Japan)
2. Tokai University School of Medicine, Isehara, Kanagawa 259-1193 (Japan)
3. Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506 (Japan)
4. Japan Synchrotron Radiation Research Institute (JASRI/SPring-8), Sayo, Hyogo 679-5198 (Japan)

Biological objects are composed of submicrometer structures such as cells and organelles that are essential for their functions. Here, we report on three-dimensional X-ray visualization of cells and organelles at resolutions up to 100 nm by imaging microtomography (micro-CT) equipped with Fresnel zone plate optics. Human cerebral tissue, fruit fly cephalic ganglia, and Escherichia coli bacteria labeled with high atomic-number elements were embedded in epoxy resin and subjected to X-ray microtomography at the BL37XU and BL47XU beamlines of the SPring-8 synchrotron radiation facility. The obtained results indicated that soft tissue structures can be visualized with the imaging microtomography.

• OSTI ID: 22494375
• Journal Information: AIP Conference Proceedings, Vol. 1696, Issue 1; Conference: XRM 2014: 12. international conference on X-ray microscopy, Melbourne (Australia), 26-31 Oct 2014; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
ANIMAL TISSUES
BIOMEDICAL RADIOGRAPHY
BRAIN
CELL CONSTITUENTS
COMPUTERIZED TOMOGRAPHY
EPOXIDES
ESCHERICHIA COLI
HUMAN POPULATIONS
RESINS
RESOLUTION
SPRING-8 STORAGE RING
SYNCHROTRON RADIATION
THREE-DIMENSIONAL CALCULATIONS
X RADIATION