Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery
Abstract
Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquid flow conservation. Finally, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.
- Authors:
-
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestraße 85, 22607 Hamburg (Germany)
- Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg (Germany)
- Helmholtz-Zentrum Geesthacht, Max-Planck-Straße 1, 21502 Geesthacht (Germany)
- Publication Date:
- OSTI Identifier:
- 22482669
- Resource Type:
- Journal Article
- Journal Name:
- Review of Scientific Instruments
- Additional Journal Information:
- Journal Volume: 86; Journal Issue: 12; Other Information: (c) 2015 Author(s); Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0034-6748
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; CERAMICS; CRYSTALLOGRAPHY; FREE ELECTRON LASERS; GAS FLOW; LIQUIDS; NOZZLES; RADIATION EFFECTS; X RADIATION
Citation Formats
Beyerlein, K. R., Heymann, M., Kirian, R., Adriano, L., Bajt, S., E-mail: sasa.bajt@desy.de, Knoška, J., Wilde, F., Chapman, H. N., Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg, and Centre for Ultrafast Imaging, Notkestraße 85, 22607 Hamburg. Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. United States: N. p., 2015.
Web. doi:10.1063/1.4936843.
Beyerlein, K. R., Heymann, M., Kirian, R., Adriano, L., Bajt, S., E-mail: sasa.bajt@desy.de, Knoška, J., Wilde, F., Chapman, H. N., Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg, & Centre for Ultrafast Imaging, Notkestraße 85, 22607 Hamburg. Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. United States. https://doi.org/10.1063/1.4936843
Beyerlein, K. R., Heymann, M., Kirian, R., Adriano, L., Bajt, S., E-mail: sasa.bajt@desy.de, Knoška, J., Wilde, F., Chapman, H. N., Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg, and Centre for Ultrafast Imaging, Notkestraße 85, 22607 Hamburg. 2015.
"Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery". United States. https://doi.org/10.1063/1.4936843.
@article{osti_22482669,
title = {Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery},
author = {Beyerlein, K. R. and Heymann, M. and Kirian, R. and Adriano, L. and Bajt, S., E-mail: sasa.bajt@desy.de and Knoška, J. and Wilde, F. and Chapman, H. N. and Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg and Centre for Ultrafast Imaging, Notkestraße 85, 22607 Hamburg},
abstractNote = {Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquid flow conservation. Finally, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.},
doi = {10.1063/1.4936843},
url = {https://www.osti.gov/biblio/22482669},
journal = {Review of Scientific Instruments},
issn = {0034-6748},
number = 12,
volume = 86,
place = {United States},
year = {Tue Dec 15 00:00:00 EST 2015},
month = {Tue Dec 15 00:00:00 EST 2015}
}
Works referenced in this record:
Modes in flow focusing and instability of coaxial liquid–gas jets
journal, June 2009
- Si, Ting; Li, Fang; Yin, Xie-Yuan
- Journal of Fluid Mechanics, Vol. 629
Potential for biomolecular imaging with femtosecond X-ray pulses
journal, August 2000
- Neutze, Richard; Wouts, Remco; van der Spoel, David
- Nature, Vol. 406, Issue 6797
Femtosecond X-ray protein nanocrystallography
journal, February 2011
- Chapman, Henry N.; Fromme, Petra; Barty, Anton
- Nature, Vol. 470, Issue 7332, p. 73-77
De novo protein crystal structure determination from X-ray free-electron laser data
journal, November 2013
- Barends, Thomas R. M.; Foucar, Lutz; Botha, Sabine
- Nature, Vol. 505, Issue 7482
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser
journal, July 2014
- Kupitz, Christopher; Basu, Shibom; Grotjohann, Ingo
- Nature, Vol. 513, Issue 7517
Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses
journal, November 2014
- Suga, Michihiro; Akita, Fusamichi; Hirata, Kunio
- Nature, Vol. 517, Issue 7532
Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography
journal, December 2013
- Johansson, Linda C.; Arnlund, David; Katona, Gergely
- Nature Communications, Vol. 4, Issue 1
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
journal, February 2014
- Weierstall, Uwe; James, Daniel; Wang, Chong
- Nature Communications, Vol. 5, Issue 1
Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
journal, August 2014
- Arnlund, David; Johansson, Linda C.; Wickstrand, Cecilia
- Nature Methods, Vol. 11, Issue 9
Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
journal, December 2011
- Barty, Anton; Caleman, Carl; Aquila, Andrew
- Nature Photonics, Vol. 6, Issue 1
Microfluidic liquid jet system with compatibility for atmospheric and high-vacuum conditions
journal, January 2014
- Trebbin, Martin; Krüger, Kilian; DePonte, Daniel
- Lab Chip, Vol. 14, Issue 10
Global and local instability of flow focusing: The influence of the geometry
journal, June 2010
- Vega, Emilio J.; Montanero, José M.; Herrada, Miguel A.
- Physics of Fluids, Vol. 22, Issue 6
On the validity of a universal solution for viscous capillary jets
journal, December 2011
- Montanero, J. M.; Herrada, M. A.; Ferrera, C.
- Physics of Fluids, Vol. 23, Issue 12
Injector for scattering measurements on fully solvated biospecies
journal, March 2012
- Weierstall, U.; Spence, J. C. H.; Doak, R. B.
- Review of Scientific Instruments, Vol. 83, Issue 3
Gas dynamic virtual nozzle for generation of microscopic droplet streams
journal, September 2008
- DePonte, D. P.; Weierstall, U.; Schmidt, K.
- Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505
Micrometer glass nozzles for flow focusing
journal, June 2010
- Montanero, J. M.; Gañán-Calvo, A. M.; Acero, A. J.
- Journal of Micromechanics and Microengineering, Vol. 20, Issue 7
Focusing liquid microjets with nozzles
journal, May 2012
- Acero, A. J.; Ferrera, C.; Montanero, J. M.
- Journal of Micromechanics and Microengineering, Vol. 22, Issue 6
The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS)
journal, March 2010
- Boutet, Sébastien; J. Williams, Garth
- New Journal of Physics, Vol. 12, Issue 3
Liquid sample delivery techniques for serial femtosecond crystallography
journal, July 2014
- Weierstall, Uwe
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser
journal, December 2011
- Lomb, Lukas; Barends, Thomas R. M.; Kassemeyer, Stephan
- Physical Review B, Vol. 84, Issue 21
Revision of capillary cone-jet physics: Electrospray and flow focusing
journal, June 2009
- Gañán-Calvo, Alfonso M.; Montanero, José M.
- Physical Review E, Vol. 79, Issue 6
Global stability of the focusing effect of fluid jet flows
journal, March 2011
- Montanero, J. M.; Rebollo-Muñoz, N.; Herrada, M. A.
- Physical Review E, Vol. 83, Issue 3
Generation of Steady Liquid Microthreads and Micron-Sized Monodisperse Sprays in Gas Streams
journal, January 1998
- Gañán-Calvo, Alfonso M.
- Physical Review Letters, Vol. 80, Issue 2
Room-temperature macromolecular serial crystallography using synchrotron radiation
journal, May 2014
- Stellato, Francesco; Oberthür, Dominik; Liang, Mengning
- IUCrJ, Vol. 1, Issue 4, p. 204-212
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
journal, May 2012
- Boutet, S.; Lomb, L.; Williams, G. J.
- Science, Vol. 337, Issue 6092
Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser
journal, November 2012
- Redecke, L.; Nass, K.; DePonte, D. P.
- Science, Vol. 339, Issue 6116
Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature
journal, February 2013
- Kern, J.; Alonso-Mori, R.; Tran, R.
- Science, Vol. 340, Issue 6131
Serial Femtosecond Crystallography of G Protein-Coupled Receptors
journal, December 2013
- Liu, W.; Wacker, D.; Gati, C.
- Science, Vol. 342, Issue 6165
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
journal, December 2014
- Tenboer, Jason; Basu, Shibom; Zatsepin, Nadia
- Science, Vol. 346, Issue 6214
Works referencing / citing this record:
Simulation of liquid micro-jet in free expanding high-speed co-flowing gas streams
journal, August 2018
- Zahoor, Rizwan; Belšak, Grega; Bajt, Saša
- Microfluidics and Nanofluidics, Vol. 22, Issue 8
Ultracompact 3D microfluidics for time-resolved structural biology
journal, January 2020
- Knoška, Juraj; Adriano, Luigi; Awel, Salah
- Nature Communications, Vol. 11, Issue 1
Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers
journal, August 2016
- Calvey, George D.; Katz, Andrea M.; Schaffer, Chris B.
- Structural Dynamics, Vol. 3, Issue 5
3D printed nozzles on a silicon fluidic chip
journal, March 2019
- Bohne, Sven; Heymann, Michael; Chapman, Henry N.
- Review of Scientific Instruments, Vol. 90, Issue 3
Controlled beams of shock-frozen, isolated, biological and artificial nanoparticles
journal, March 2020
- Samanta, Amit K.; Amin, Muhamed; Estillore, Armando D.
- Structural Dynamics, Vol. 7, Issue 2
3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating
journal, August 2019
- Echelmeier, Austin; Kim, Daihyun; Cruz Villarreal, Jorvani
- Journal of Applied Crystallography, Vol. 52, Issue 5
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography
journal, August 2018
- Botha, S.; Baitan, D.; Jungnickel, K. E. J.
- IUCrJ, Vol. 5, Issue 5
Sample delivery for serial crystallography at free-electron lasers and synchrotrons
journal, January 2019
- Grünbein, Marie Luise; Nass Kovacs, Gabriela
- Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
A guide to sample delivery systems for serial crystallography
journal, August 2019
- Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai
- The FEBS Journal, Vol. 286, Issue 22
Three-dimensional-printed gas dynamic virtual nozzles for x-ray laser sample delivery
journal, January 2016
- Nelson, Garrett; Kirian, Richard A.; Weierstall, Uwe
- Optics Express, Vol. 24, Issue 11
Light-sheet imaging for the recording of transverse absolute density distributions of gas-phase particle-beams from nanoparticle injectors
journal, January 2019
- Worbs, Lena; Lübke, Jannik; Roth, Nils
- Optics Express, Vol. 27, Issue 25
Jet delivery system for Raman scattering on bio-inorganic compounds
text, January 2016
- Wetzel, Alina; Biebl, F.; Beyerlein, K. R.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Rapid Sample Delivery for Megahertz Serial Crystallography at X-ray FELs
text, January 2018
- Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Megahertz serial crystallography
text, January 2018
- Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Double-flow focused liquid injector for efficient serial femtosecond crystallography
journal, March 2017
- Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O.
- Scientific Reports, Vol. 7, Issue 1
Jet delivery system for Raman scattering on bio-inorganic compounds
journal, November 2016
- Wetzel, A.; Biebl, F.; Beyerlein, K. R.
- Applied Physics Letters, Vol. 109, Issue 21
Rapid sample delivery for megahertz serial crystallography at X-ray FELs
journal, July 2018
- Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J.
- IUCrJ, Vol. 5, Issue 5
3D printed nozzles on a silicon fluidic chip
text, January 2019
- Bohne, Sven; Heymann, Michael; Chapman, Henry N.
- American Institute of Physics
Ultracompact 3D microfluidics for time-resolved structural biology
text, January 2020
- Knoška, Juraj; Adriano, Luigi; Awel, Salah
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Megahertz serial crystallography
journal, October 2018
- Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard
- Nature Communications, Vol. 9, Issue 1
Rapid sample delivery for megahertz serial crystallography at X-ray FELs
text, January 2018
- Wiedorn, Max Oliver; Awel, Salah; Morgan, Andrew J.
- TUHH Universitätsbibliothek
Ultracompact 3D microfluidics for time-resolved structural biology
text, January 2020
- Knoska, Juraj; Adriano, Luigi; Awel, Salah
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Megahertz serial crystallography
text, January 2018
- Wiedorn, Max Oliver; Oberthür, Dominik; Bean, Richard
- Nature Publishing Group UK
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography
text, January 2018
- Botha, S.; Baitan, D.; Jungnickel, K. E. J.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Light-sheet imaging for the recording of transverse absolute density distributions of gas-phase particle-beams from nanoparticle injectors
text, January 2019
- Worbs, Lena; Lübke, Jannik; Roth, Nils
- arXiv
Ultracompact 3D microfluidics for time-resolved structural biology
journal, January 2020
- Knoška, Juraj; Adriano, Luigi; Awel, Salah
- Nature Communications, Vol. 11, Issue 1
Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers
journal, August 2016
- Calvey, George D.; Katz, Andrea M.; Schaffer, Chris B.
- Structural Dynamics, Vol. 3, Issue 5
Controlled beams of shock-frozen, isolated, biological and artificial nanoparticles
text, January 2020
- Amin, Muhamed; Estillore, Armando; Roth, Nils
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Laser-induced acoustic desorption of thermally stable and unstable biomolecules
preprint, January 2018
- Huang, Zhipeng; Horke, Daniel A.; Küpper, Jochen
- arXiv
Dripping, jetting and tip streaming
preprint, January 2019
- Montanero, Jose M.; Ganan-Calvo, Alfonso M.
- arXiv
Light-sheet imaging for the recording of transverse absolute density distributions of gas-phase particle-beams from nanoparticle injectors
text, January 2019
- Worbs, Lena; Lübke, Jannik; Roth, Nils
- arXiv
Controlled beams of shockfrozen, isolated, biological and artificial nanoparticles
preprint, January 2019
- Samanta, Amit K.; Amin, Muhamed; Estillore, Armando D.
- arXiv