skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Improving z-tracking accuracy in the two-photon single-particle tracking microscope

Abstract

Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simulations show that the MLE-based tracking scheme can improve the z-tracking accuracy of TSUNAMI microscope by 1.7 fold. In addition, MLE is also found to reduce the temporal correlation of the z-tracking error. Taking advantage of the smaller and less temporally correlated z-tracking error, we have precisely recovered the hybridization-melting kinetics of a DNA model system from thousands of short single-particle trajectories in silico. Our method can be generally applied to other 3D single-particle tracking techniques.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22482259
Resource Type:
Journal Article
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 107; Journal Issue: 15; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0003-6951
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ACCURACY; COMPUTERIZED SIMULATION; CORRELATIONS; DNA; ERRORS; MAXIMUM-LIKELIHOOD FIT; MELTING; MICROSCOPES; MONTE CARLO METHOD; NONLINEAR PROBLEMS; PHOTONS; TRAJECTORIES

Citation Formats

Liu, C., Liu, Y. -L., Perillo, E. P., Jiang, N., Dunn, A. K., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu, and Yeh, H.-C., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu. Improving z-tracking accuracy in the two-photon single-particle tracking microscope. United States: N. p., 2015. Web. doi:10.1063/1.4932224.
Liu, C., Liu, Y. -L., Perillo, E. P., Jiang, N., Dunn, A. K., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu, & Yeh, H.-C., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu. Improving z-tracking accuracy in the two-photon single-particle tracking microscope. United States. https://doi.org/10.1063/1.4932224
Liu, C., Liu, Y. -L., Perillo, E. P., Jiang, N., Dunn, A. K., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu, and Yeh, H.-C., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu. 2015. "Improving z-tracking accuracy in the two-photon single-particle tracking microscope". United States. https://doi.org/10.1063/1.4932224.
@article{osti_22482259,
title = {Improving z-tracking accuracy in the two-photon single-particle tracking microscope},
author = {Liu, C. and Liu, Y. -L. and Perillo, E. P. and Jiang, N. and Dunn, A. K., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu and Yeh, H.-C., E-mail: adunn@utexas.edu, E-mail: tim.yeh@austin.utexas.edu},
abstractNote = {Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simulations show that the MLE-based tracking scheme can improve the z-tracking accuracy of TSUNAMI microscope by 1.7 fold. In addition, MLE is also found to reduce the temporal correlation of the z-tracking error. Taking advantage of the smaller and less temporally correlated z-tracking error, we have precisely recovered the hybridization-melting kinetics of a DNA model system from thousands of short single-particle trajectories in silico. Our method can be generally applied to other 3D single-particle tracking techniques.},
doi = {10.1063/1.4932224},
url = {https://www.osti.gov/biblio/22482259}, journal = {Applied Physics Letters},
issn = {0003-6951},
number = 15,
volume = 107,
place = {United States},
year = {Mon Oct 12 00:00:00 EDT 2015},
month = {Mon Oct 12 00:00:00 EDT 2015}
}