The effects of in utero bisphenol A exposure on reproductive capacity in several generations of mice

Ziv-Gal, Ayelet; Wang, Wei; Zhou, Changqing

doi:10.1016/J.TAAP.2015.03.003

Title: The effects of in utero bisphenol A exposure on reproductive capacity in several generations of mice

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Abstract

In utero bisphenol A (BPA) exposure affects reproductive function in the first generation (F1) of mice; however, not many studies have examined the reproductive effects of BPA exposure on subsequent generations. In this study, pregnant mice (F0) were orally dosed with vehicle, BPA (0.5, 20, and 50 μg/kg/day) or diethylstilbestrol (DES; 0.05 μg/kg/day) daily from gestation day 11 until birth. F1 females were used to generate the F2 generation, and F2 females were used to generate the F3 generation. Breeding studies at the ages of 3, 6, and 9 months were conducted to evaluate reproductive capacity over time. Further, studies were conducted to evaluate pubertal onset, litter size, and percentage of dead pups; and to calculate pregnancy rate, and mating, fertility, and gestational indices. The results indicate that BPA exposure (0.5 and 50 μg/kg/day) significantly delayed the age at vaginal opening in the F3 generation compared to vehicle control. Both DES (0.05 μg/kg/day) and BPA (50 μg/kg/day) significantly delayed the age at first estrus in the F3 generation compared to vehicle control. BPA exposure reduced gestational index in the F1 and F2 generations compared to control. Further, BPA exposure (0.5 μg/kg/day) compromised the fertility index in the F3 generation comparedmore »« less

Authors:: Ziv-Gal, Ayelet; Wang, Wei; Zhou, Changqing

Publication Date:: Fri May 01 00:00:00 EDT 2015

OSTI Identifier:: 22465753

Resource Type:: Journal Article

Journal Name:: Toxicology and Applied Pharmacology

Additional Journal Information:: Journal Volume: 284; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; BREEDING; COMPARATIVE EVALUATIONS; FEMALES; FERTILITY; LITTER SIZE; MATING; MICE; OPENINGS; PARTURITION; PREGNANCY

Citation Formats


                    Ziv-Gal, Ayelet, Wang, Wei, Zhou, Changqing, and Flaws, Jodi A., E-mail: jflaws@illinois.edu. The effects of in utero bisphenol A exposure on reproductive capacity in several generations of mice.  United States: N. p., 2015. 
        Web.  doi:10.1016/J.TAAP.2015.03.003.

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                    Ziv-Gal, Ayelet, Wang, Wei, Zhou, Changqing, & Flaws, Jodi A., E-mail: jflaws@illinois.edu. The effects of in utero bisphenol A exposure on reproductive capacity in several generations of mice.  United States.  https://doi.org/10.1016/J.TAAP.2015.03.003

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                    Ziv-Gal, Ayelet, Wang, Wei, Zhou, Changqing, and Flaws, Jodi A., E-mail: jflaws@illinois.edu. 2015.  
        "The effects of in utero bisphenol A exposure on reproductive capacity in several generations of mice".  United States.  https://doi.org/10.1016/J.TAAP.2015.03.003.

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@article{osti_22465753,

  title        = {The effects of in utero bisphenol A exposure on reproductive capacity in several generations of mice},

  author       = {Ziv-Gal, Ayelet and Wang, Wei and Zhou, Changqing and Flaws, Jodi A., E-mail: jflaws@illinois.edu},

  abstractNote = {In utero bisphenol A (BPA) exposure affects reproductive function in the first generation (F1) of mice; however, not many studies have examined the reproductive effects of BPA exposure on subsequent generations. In this study, pregnant mice (F0) were orally dosed with vehicle, BPA (0.5, 20, and 50 μg/kg/day) or diethylstilbestrol (DES; 0.05 μg/kg/day) daily from gestation day 11 until birth. F1 females were used to generate the F2 generation, and F2 females were used to generate the F3 generation. Breeding studies at the ages of 3, 6, and 9 months were conducted to evaluate reproductive capacity over time. Further, studies were conducted to evaluate pubertal onset, litter size, and percentage of dead pups; and to calculate pregnancy rate, and mating, fertility, and gestational indices. The results indicate that BPA exposure (0.5 and 50 μg/kg/day) significantly delayed the age at vaginal opening in the F3 generation compared to vehicle control. Both DES (0.05 μg/kg/day) and BPA (50 μg/kg/day) significantly delayed the age at first estrus in the F3 generation compared to vehicle control. BPA exposure reduced gestational index in the F1 and F2 generations compared to control. Further, BPA exposure (0.5 μg/kg/day) compromised the fertility index in the F3 generation compared to control. Finally, in utero BPA exposure reduced the ability of female mice to maintain pregnancies as they aged. Collectively, these data suggest that BPA exposure affects reproductive function in female mice and that some effects may be transgenerational in nature. - Highlights: • In utero BPA delayed vaginal opening in the F3 generation compared to control. • In utero BPA delayed estrus in the F3 generation compared to control. • In utero BPA reduced the ability of F1 and F2 female mice to maintain pregnancies. • In utero BPA compromised the ability of F3 female mice to become pregnant. • Some effects of in utero BPA may be transgenerational in nature.},

  doi          = {10.1016/J.TAAP.2015.03.003},

  url          = {https://www.osti.gov/biblio/22465753},
  journal      = {Toxicology and Applied Pharmacology},

  issn         = {0041-008X},

  number       = 3,

  volume       = 284,

  place        = {United States},

  year         = {Fri May 01 00:00:00 EDT 2015},

  month        = {Fri May 01 00:00:00 EDT 2015}

}

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