miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma
Abstract
Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights:more »
- Authors:
-
- Department II of Interventional Radiology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438 (China)
- Department II of Special Medical Care, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438 (China)
- Publication Date:
- OSTI Identifier:
- 22462181
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 464; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; APOPTOSIS; CELL PROLIFERATION; HEPATOMAS; IN VITRO; INHIBITION; LARGE INTESTINE; LUNGS; METASTASES; PHENOTYPE; PLANT GROWTH; RNA; TUMOR CELLS
Citation Formats
Zhai, Jian, Qu, Shuping, Li, Xiaowei, Zhong, Jiaming, Chen, Xiaoxia, Qu, Zengqiang, and Wu, Dong. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma. United States: N. p., 2015.
Web. doi:10.1016/J.BBRC.2015.06.108.
Zhai, Jian, Qu, Shuping, Li, Xiaowei, Zhong, Jiaming, Chen, Xiaoxia, Qu, Zengqiang, & Wu, Dong. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma. United States. https://doi.org/10.1016/J.BBRC.2015.06.108
Zhai, Jian, Qu, Shuping, Li, Xiaowei, Zhong, Jiaming, Chen, Xiaoxia, Qu, Zengqiang, and Wu, Dong. 2015.
"miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma". United States. https://doi.org/10.1016/J.BBRC.2015.06.108.
@article{osti_22462181,
title = {miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma},
author = {Zhai, Jian and Qu, Shuping and Li, Xiaowei and Zhong, Jiaming and Chen, Xiaoxia and Qu, Zengqiang and Wu, Dong},
abstractNote = {Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights: • Decreased of miR-129 is found in HCC and associated with advanced stage and metastasis. • miR-129 suppresses proliferation and invasion of HCC cells. • miR-129 directly targets the 3′ UTR of PAK5 and diminishes PAK5 expression. • PAK5 is involved in miR-129 mediated suppression functions.},
doi = {10.1016/J.BBRC.2015.06.108},
url = {https://www.osti.gov/biblio/22462181},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 464,
place = {United States},
year = {Fri Aug 14 00:00:00 EDT 2015},
month = {Fri Aug 14 00:00:00 EDT 2015}
}