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Title: Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues

Abstract

Adipose tissue growth is associated with preadipocyte proliferation and differentiation. Telomere length is a biological marker for cell proliferation. Preadipocyte factor-1 (pref-1) is specifically expressed in preadipocytes and acts as a molecular gatekeeper of adipogenesis. In the present study, we investigated the fat depot-specific differences in telomere length and pref-1 gene expression in various anatomical sites (subcutaneous, intramuscular and visceral) of fattening Wagyu cattle. Visceral adipose tissue expressed higher pref-1 mRNA than did subcutaneous and intramuscular adipose tissues. The telomere length in visceral adipose tissue tended to be longer than that of subcutaneous and intramuscular adipose tissues. The telomere length of adipose tissue was not associated with adipocyte size from three anatomical sites. No significant correlation was found between the pref-1 mRNA level and the subcutaneous adipocyte size. In contrast, the pref-1 mRNA level was negatively correlated with the intramuscular and visceral adipocyte size. These results suggest that anatomical sites of adipose tissue affect the telomere length and expression pattern of the pref-1 gene in a fat depot-specific manner. - Highlights: • Visceral adipose tissue express higher pref-1 mRNA than other anatomical sites. • Telomere length in visceral adipose tissue is longer than other anatomical sites. • Telomere length ofmore » adipose tissue is not associated with adipocyte size. • Pref-1 mRNA is negatively correlated with intramuscular and visceral adipocyte size.« less

Authors:
; ;
Publication Date:
OSTI Identifier:
22462154
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 463; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; BIOLOGICAL MARKERS; CATTLE; CELL PROLIFERATION; FATS; GENES; LENGTH; MESSENGER-RNA; PLANT GROWTH; PROTEINS

Citation Formats

Yamada, Tomoya, Higuchi, Mikito, and Nakanishi, Naoto. Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.06.036.
Yamada, Tomoya, Higuchi, Mikito, & Nakanishi, Naoto. Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues. United States. https://doi.org/10.1016/J.BBRC.2015.06.036
Yamada, Tomoya, Higuchi, Mikito, and Nakanishi, Naoto. 2015. "Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues". United States. https://doi.org/10.1016/J.BBRC.2015.06.036.
@article{osti_22462154,
title = {Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues},
author = {Yamada, Tomoya and Higuchi, Mikito and Nakanishi, Naoto},
abstractNote = {Adipose tissue growth is associated with preadipocyte proliferation and differentiation. Telomere length is a biological marker for cell proliferation. Preadipocyte factor-1 (pref-1) is specifically expressed in preadipocytes and acts as a molecular gatekeeper of adipogenesis. In the present study, we investigated the fat depot-specific differences in telomere length and pref-1 gene expression in various anatomical sites (subcutaneous, intramuscular and visceral) of fattening Wagyu cattle. Visceral adipose tissue expressed higher pref-1 mRNA than did subcutaneous and intramuscular adipose tissues. The telomere length in visceral adipose tissue tended to be longer than that of subcutaneous and intramuscular adipose tissues. The telomere length of adipose tissue was not associated with adipocyte size from three anatomical sites. No significant correlation was found between the pref-1 mRNA level and the subcutaneous adipocyte size. In contrast, the pref-1 mRNA level was negatively correlated with the intramuscular and visceral adipocyte size. These results suggest that anatomical sites of adipose tissue affect the telomere length and expression pattern of the pref-1 gene in a fat depot-specific manner. - Highlights: • Visceral adipose tissue express higher pref-1 mRNA than other anatomical sites. • Telomere length in visceral adipose tissue is longer than other anatomical sites. • Telomere length of adipose tissue is not associated with adipocyte size. • Pref-1 mRNA is negatively correlated with intramuscular and visceral adipocyte size.},
doi = {10.1016/J.BBRC.2015.06.036},
url = {https://www.osti.gov/biblio/22462154}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 463,
place = {United States},
year = {Fri Aug 07 00:00:00 EDT 2015},
month = {Fri Aug 07 00:00:00 EDT 2015}
}