Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy

Journal Article · Fri Feb 06 00:00:00 EST 2015 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2014.12.091· OSTI ID:22458469

Xiao, Xiao ^[1]; Gang, Yi ^[1]; Wang, Honghong ^[2]; Wang, Jiayin ^[3]; Zhao, Lina ^[4]; Xu, Li ^[1]; Liu, Zhiguo ^[1]

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1. State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province (China)
2. No. 518 Hospital of Chinese People’s Liberation Army, Xi’an 710043, Shaanxi Province (China)
3. The Genome Institute, Washington University in St. Louis, St. Louis, MO 63108 (United States)
4. Department of Radiation Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province (China)

Highlights: • A shRNA vector based transcription factor decoy, VB-ODN, was designed. • VB-ODN for NF-κB inhibited cell viability in HEK293 cells. • VB-ODN inhibited expression of downstream genes of target transcription factors. • VB-ODN may enhance nuclear entry ratio for its feasibility of virus production. - Abstract: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.

• OSTI ID: 22458469
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 457, Issue 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BROMIDES
DECARBOXYLASES
DESIGN
ELECTROPHORESIS
GENES
GROWTH FACTORS
HUMAN POPULATIONS
KIDNEYS
MONOCYTES
ORNITHINE
RECEPTORS
RNA
TETRAZOLIUM
TRANSCRIPTION FACTORS
VIABILITY
VIRUSES
VITAMIN D