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Title: miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

Abstract

A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.

Authors:
 [1]
  1. Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL (United Kingdom)
Publication Date:
OSTI Identifier:
22435005
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 448; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BALANCES; CELL PROLIFERATION; HUMAN POPULATIONS; PROTEINS; VIRUSES

Citation Formats

McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, Patel, Daksha, and McCance, Dennis J., E-mail: d.mccance@qub.ac.uk. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes. United States: N. p., 2014. Web. doi:10.1016/J.VIROL.2013.10.014.
McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, Patel, Daksha, & McCance, Dennis J., E-mail: d.mccance@qub.ac.uk. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes. United States. https://doi.org/10.1016/J.VIROL.2013.10.014
McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, Patel, Daksha, and McCance, Dennis J., E-mail: d.mccance@qub.ac.uk. 2014. "miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes". United States. https://doi.org/10.1016/J.VIROL.2013.10.014.
@article{osti_22435005,
title = {miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes},
author = {McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk and Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL and Patel, Daksha and McCance, Dennis J., E-mail: d.mccance@qub.ac.uk},
abstractNote = {A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.},
doi = {10.1016/J.VIROL.2013.10.014},
url = {https://www.osti.gov/biblio/22435005}, journal = {Virology},
issn = {0042-6822},
number = ,
volume = 448,
place = {United States},
year = {Sun Jan 05 00:00:00 EST 2014},
month = {Sun Jan 05 00:00:00 EST 2014}
}