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Title: Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression

Abstract

Highlights: • miR-9 expression level was significantly decreased in OSCC tissues. • Curcumin significantly inhibited SCC-9 cells proliferation. • miR-9 mediates the inhibition of SCC-9 proliferation by curcumin. • Curcumin suppresses Wnt/β-catenin signaling in SCC-9 cells. • miR-9 mediates the suppression of Wnt/β-catenin signaling by curcumin. - Abstract: Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells,more » but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future.« less

Authors:
 [1]; ;  [2];  [3];  [1]
  1. Department of Occupational Medicine and Environmental Health, School of Public Health, Soochow University, Suzhou 215123 (China)
  2. Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006 (China)
  3. Department of Head and Neck Tumors, Shanghai Ninth People’s Hospital Affiliated Shanghai JiaoTong University School of Medicine, Shanghai 200011 (China)
Publication Date:
OSTI Identifier:
22416848
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 454; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CARCINOMAS; CELL PROLIFERATION; CURCUMIN; IN VITRO; INHIBITION; OLIGONUCLEOTIDES; RNA; TUMOR CELLS

Citation Formats

Xiao, Can, Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Wang, Lili, Zhu, Lifang, Zhang, Chenping, and Zhou, Jianhua. Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.10.122.
Xiao, Can, Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Wang, Lili, Zhu, Lifang, Zhang, Chenping, & Zhou, Jianhua. Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression. United States. https://doi.org/10.1016/J.BBRC.2014.10.122
Xiao, Can, Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Wang, Lili, Zhu, Lifang, Zhang, Chenping, and Zhou, Jianhua. 2014. "Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression". United States. https://doi.org/10.1016/J.BBRC.2014.10.122.
@article{osti_22416848,
title = {Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression},
author = {Xiao, Can and Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006 and Wang, Lili and Zhu, Lifang and Zhang, Chenping and Zhou, Jianhua},
abstractNote = {Highlights: • miR-9 expression level was significantly decreased in OSCC tissues. • Curcumin significantly inhibited SCC-9 cells proliferation. • miR-9 mediates the inhibition of SCC-9 proliferation by curcumin. • Curcumin suppresses Wnt/β-catenin signaling in SCC-9 cells. • miR-9 mediates the suppression of Wnt/β-catenin signaling by curcumin. - Abstract: Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells, but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future.},
doi = {10.1016/J.BBRC.2014.10.122},
url = {https://www.osti.gov/biblio/22416848}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 454,
place = {United States},
year = {Fri Nov 28 00:00:00 EST 2014},
month = {Fri Nov 28 00:00:00 EST 2014}
}