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Title: Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo

Abstract

Highlights: • Gene and cell-based therapies comprise innovative aspects of regenerative medicine. • Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS. • Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords. • Over-expressing oct4 and sox2 also induced production of neural marker PGP9.5. • Formation of new nerve cells, secreting trophic factors and neo-vascularisation could improve symptoms in ALS. - Abstract: Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.

Authors:
 [1]; ; ;  [2];  [2];  [3]
  1. Kazan State Medical University, Kazan, Republic of Tatarstan (Russian Federation)
  2. Kazan Federal University, Kazan, Republic of Tatarstan (Russian Federation)
  3. Private Medical Practice and Research Center; Hungary
Publication Date:
OSTI Identifier:
22416727
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 451; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLOOD CELLS; DRUGS; GENES; HUMAN POPULATIONS; IN VIVO; MICE; MUSCLES; NERVE CELLS; SPINAL CORD; STEM CELLS; SYMPTOMS; THERAPY; TRANSCRIPTION FACTORS; TRANSPLANTS

Citation Formats

Guseva, Daria, Hannover Medical School, Hannover, Rizvanov, Albert A., Salafutdinov, Ilnur I., Kudryashova, Nezhdana V., Palotás, András, Asklepios-Med, Szeged, and Islamov, Rustem R., E-mail: islamru@yahoo.com. Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.07.132.
Guseva, Daria, Hannover Medical School, Hannover, Rizvanov, Albert A., Salafutdinov, Ilnur I., Kudryashova, Nezhdana V., Palotás, András, Asklepios-Med, Szeged, & Islamov, Rustem R., E-mail: islamru@yahoo.com. Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo. United States. https://doi.org/10.1016/J.BBRC.2014.07.132
Guseva, Daria, Hannover Medical School, Hannover, Rizvanov, Albert A., Salafutdinov, Ilnur I., Kudryashova, Nezhdana V., Palotás, András, Asklepios-Med, Szeged, and Islamov, Rustem R., E-mail: islamru@yahoo.com. 2014. "Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo". United States. https://doi.org/10.1016/J.BBRC.2014.07.132.
@article{osti_22416727,
title = {Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo},
author = {Guseva, Daria and Hannover Medical School, Hannover and Rizvanov, Albert A. and Salafutdinov, Ilnur I. and Kudryashova, Nezhdana V. and Palotás, András and Asklepios-Med, Szeged and Islamov, Rustem R., E-mail: islamru@yahoo.com},
abstractNote = {Highlights: • Gene and cell-based therapies comprise innovative aspects of regenerative medicine. • Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS. • Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords. • Over-expressing oct4 and sox2 also induced production of neural marker PGP9.5. • Formation of new nerve cells, secreting trophic factors and neo-vascularisation could improve symptoms in ALS. - Abstract: Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.},
doi = {10.1016/J.BBRC.2014.07.132},
url = {https://www.osti.gov/biblio/22416727}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 451,
place = {United States},
year = {Fri Sep 05 00:00:00 EDT 2014},
month = {Fri Sep 05 00:00:00 EDT 2014}
}