Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension

Shao, Dongmin; Perros, Frédéric; Caramori, Gaetano; Meng, Chao; Dormuller, Peter; Chou, Pai-Chien; Church, Colin; Papi, Alberto; Casolari, Paolo; Welsh, David; Peacock, Andrew; Humbert, Marc; Adcock, Ian M.

doi:10.1016/J.BBRC.2014.06.111

Title: Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension

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Abstract

Highlights: • Nuclear IL-33 expression is reduced in vascular endothelial cells from PAH patients. • Knockdown of IL-33 leads to increased IL-6 and sST2 mRNA expression. • IL-33 binds homeobox motifs in target gene promoters and recruits repressor proteins. - Abstract: Idiopathic pulmonary arterial hypertension (IPAH) is an incurable condition leading to right ventricular failure and death and inflammation is postulated to be associated with vascular remodelling. Interleukin (IL)-33, a member of the “alarmin” family can either act on the membrane ST2 receptor or as a nuclear repressor, to regulate inflammation. We show, using immunohistochemistry, that IL-33 expression is nuclear in the vessels of healthy subjects whereas nuclear IL-33 is markedly diminished in the vessels of IPAH patients. This correlates with reduced IL-33 mRNA expression in their lung. In contrast, serum levels of IL-33 are unchanged in IPAH. However, the expression of the soluble form of ST2, sST2, is enhanced in the serum of IPAH patients. Knock-down of IL-33 in human endothelial cells (ECs) using siRNA is associated with selective modulation of inflammatory genes involved in vascular remodelling including IL-6. Additionally, IL-33 knock-down significantly increased sST2 release from ECs. Chromatin immunoprecipitation demonstrated that IL-33 bound multiple putative homeodomain protein bindingmore »« less

Authors:

Shao, Dongmin ^[1]; Perros, Frédéric ^[2]; Caramori, Gaetano ^[3]; Meng, Chao ^[1]; Dormuller, Peter ^[2]; Chou, Pai-Chien ^[4]; Church, Colin ^[5]; Papi, Alberto; Casolari, Paolo ^[3]; Welsh, David; Peacock, Andrew ^[5]; Humbert, Marc ^[2]; Adcock, Ian M. ^[4]

Section of Vascular Biology, National Heart and Lung Institute, Imperial College London, London (United Kingdom)
Faculté de Médecine, Université Paris-Sud, Paris, Clamart (France)
Dipartimento di Scienze Mediche, Sezione di Medicina Interna e Cardiorespiratoria, Centro Interdipartimentale per lo Studio delle Malattie Infiammatorie delle Vie Aeree e Patologie Fumo-Correlate, University of Ferrara, Ferrara (Italy)
Airways Disease, National Heart and Lung Institute (United Kingdom)
Scottish Pulmonary Vascular Unit, University of Glasgow (United Kingdom)

Publication Date:: Fri Aug 15 00:00:00 EDT 2014

OSTI Identifier:: 22416707

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 451; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CELL MEMBRANES; CHROMATIN; COMPLEXES; DEATH; GENES; HUMAN POPULATIONS; HYPERTENSION; INFLAMMATION; LUNGS; MESSENGER-RNA; METHYL TRANSFERASES; PATHOGENESIS; PATIENTS; POLYCYCLIC AROMATIC HYDROCARBONS; RECEPTORS

Citation Formats


                    Shao, Dongmin, Perros, Frédéric, Caramori, Gaetano, Meng, Chao, Department of Geriatrics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, Dormuller, Peter, Chou, Pai-Chien, Church, Colin, Papi, Alberto, Casolari, Paolo, Welsh, David, Peacock, Andrew, Humbert, Marc, Adcock, Ian M., and Wort, Stephen J., E-mail: s.wort@imperial.ac.uk. Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension.  United States: N. p., 2014. 
        Web.  doi:10.1016/J.BBRC.2014.06.111.

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                    Shao, Dongmin, Perros, Frédéric, Caramori, Gaetano, Meng, Chao, Department of Geriatrics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, Dormuller, Peter, Chou, Pai-Chien, Church, Colin, Papi, Alberto, Casolari, Paolo, Welsh, David, Peacock, Andrew, Humbert, Marc, Adcock, Ian M., & Wort, Stephen J., E-mail: s.wort@imperial.ac.uk. Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension.  United States.  https://doi.org/10.1016/J.BBRC.2014.06.111

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                    Shao, Dongmin, Perros, Frédéric, Caramori, Gaetano, Meng, Chao, Department of Geriatrics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, Dormuller, Peter, Chou, Pai-Chien, Church, Colin, Papi, Alberto, Casolari, Paolo, Welsh, David, Peacock, Andrew, Humbert, Marc, Adcock, Ian M., and Wort, Stephen J., E-mail: s.wort@imperial.ac.uk. 2014.  
        "Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension".  United States.  https://doi.org/10.1016/J.BBRC.2014.06.111.

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@article{osti_22416707,

  title        = {Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension},

  author       = {Shao, Dongmin and Perros, Frédéric and Caramori, Gaetano and Meng, Chao and Department of Geriatrics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai and Dormuller, Peter and Chou, Pai-Chien and Church, Colin and Papi, Alberto and Casolari, Paolo and Welsh, David and Peacock, Andrew and Humbert, Marc and Adcock, Ian M. and Wort, Stephen J., E-mail: s.wort@imperial.ac.uk},

  abstractNote = {Highlights: • Nuclear IL-33 expression is reduced in vascular endothelial cells from PAH patients. • Knockdown of IL-33 leads to increased IL-6 and sST2 mRNA expression. • IL-33 binds homeobox motifs in target gene promoters and recruits repressor proteins. - Abstract: Idiopathic pulmonary arterial hypertension (IPAH) is an incurable condition leading to right ventricular failure and death and inflammation is postulated to be associated with vascular remodelling. Interleukin (IL)-33, a member of the “alarmin” family can either act on the membrane ST2 receptor or as a nuclear repressor, to regulate inflammation. We show, using immunohistochemistry, that IL-33 expression is nuclear in the vessels of healthy subjects whereas nuclear IL-33 is markedly diminished in the vessels of IPAH patients. This correlates with reduced IL-33 mRNA expression in their lung. In contrast, serum levels of IL-33 are unchanged in IPAH. However, the expression of the soluble form of ST2, sST2, is enhanced in the serum of IPAH patients. Knock-down of IL-33 in human endothelial cells (ECs) using siRNA is associated with selective modulation of inflammatory genes involved in vascular remodelling including IL-6. Additionally, IL-33 knock-down significantly increased sST2 release from ECs. Chromatin immunoprecipitation demonstrated that IL-33 bound multiple putative homeodomain protein binding motifs in the proximal and distal promoters of ST2 genes. IL-33 formed a complex with the histone methyltransferase SUV39H1, a transcriptional repressor. In conclusion, IL-33 regulates the expression of IL-6 and sST2, an endogenous IL-33 inhibitor, in primary human ECs and may play an important role in the pathogenesis of PAH through recruitment of transcriptional repressor proteins.},

  doi          = {10.1016/J.BBRC.2014.06.111},

  url          = {https://www.osti.gov/biblio/22416707},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 1,

  volume       = 451,

  place        = {United States},

  year         = {Fri Aug 15 00:00:00 EDT 2014},

  month        = {Fri Aug 15 00:00:00 EDT 2014}

}

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