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Title: Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance

Abstract

Highlights: • Genomes of a butanol tolerant strain and its parent strain were deciphered. • Comparative genomic and proteomic was applied to understand butanol tolerance. • None differentially expressed proteins have mutations in its corresponding genes. • Mutations in ribosome might be responsible for the global difference of proteomics. - Abstract: Clostridium acetobutylicum strain Rh8 is a butanol-tolerant mutant which can tolerate up to 19 g/L butanol, 46% higher than that of its parent strain DSM 1731. We previously performed comparative cytoplasm- and membrane-proteomic analyses to understand the mechanism underlying the improved butanol tolerance of strain Rh8. In this work, we further extended this comparison to the genomic level. Compared with the genome of the parent strain DSM 1731, two insertion sites, four deletion sites, and 67 single nucleotide variations (SNVs) are distributed throughout the genome of strain Rh8. Among the 67 SNVs, 16 SNVs are located in the predicted promoters and intergenic regions; while 29 SNVs are located in the coding sequence, affecting a total of 21 proteins involved in transport, cell structure, DNA replication, and protein translation. The remaining 22 SNVs are located in the ribosomal genes, affecting a total of 12 rRNA genes in different operons. Analysismore » of previous comparative proteomic data indicated that none of the differentially expressed proteins have mutations in its corresponding genes. Rchange Algorithms analysis indicated that the mutations occurred in the ribosomal genes might change the ribosome RNA thermodynamic characteristics, thus affect the translation strength of these proteins. Take together, the improved butanol tolerance of C. acetobutylicum strain Rh8 might be acquired through regulating the translational process to achieve different expression strength of genes involved in butanol tolerance.« less

Authors:
 [1]; ; ;  [1];  [1];  [1];  [2];  [1]
  1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing (China)
  2. Department of Human Genetics, School of Medicine, University of California, Los Angeles, CA 90095 (United States)
Publication Date:
OSTI Identifier:
22416701
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 450; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALGORITHMS; BUTANOLS; CELL MEMBRANES; CLOSTRIDIUM ACETOBUTYLICUM; COMPARATIVE EVALUATIONS; CYTOPLASM; DNA REPLICATION; GENES; MUTATIONS; NUCLEOTIDES; PROMOTERS; PROTEINS; RNA; STRAINS; THERMODYNAMIC PROPERTIES; TOLERANCE

Citation Formats

Bao, Guanhui, University of Chinese Academy of Sciences, Beijing, Dong, Hongjun, Zhu, Yan, Mao, Shaoming, Zhang, Tianrui, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, Zhang, Yanping, Chen, Zugen, and Li, Yin. Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.07.052.
Bao, Guanhui, University of Chinese Academy of Sciences, Beijing, Dong, Hongjun, Zhu, Yan, Mao, Shaoming, Zhang, Tianrui, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, Zhang, Yanping, Chen, Zugen, & Li, Yin. Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance. United States. https://doi.org/10.1016/J.BBRC.2014.07.052
Bao, Guanhui, University of Chinese Academy of Sciences, Beijing, Dong, Hongjun, Zhu, Yan, Mao, Shaoming, Zhang, Tianrui, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, Zhang, Yanping, Chen, Zugen, and Li, Yin. 2014. "Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance". United States. https://doi.org/10.1016/J.BBRC.2014.07.052.
@article{osti_22416701,
title = {Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance},
author = {Bao, Guanhui and University of Chinese Academy of Sciences, Beijing and Dong, Hongjun and Zhu, Yan and Mao, Shaoming and Zhang, Tianrui and Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin and Zhang, Yanping and Chen, Zugen and Li, Yin},
abstractNote = {Highlights: • Genomes of a butanol tolerant strain and its parent strain were deciphered. • Comparative genomic and proteomic was applied to understand butanol tolerance. • None differentially expressed proteins have mutations in its corresponding genes. • Mutations in ribosome might be responsible for the global difference of proteomics. - Abstract: Clostridium acetobutylicum strain Rh8 is a butanol-tolerant mutant which can tolerate up to 19 g/L butanol, 46% higher than that of its parent strain DSM 1731. We previously performed comparative cytoplasm- and membrane-proteomic analyses to understand the mechanism underlying the improved butanol tolerance of strain Rh8. In this work, we further extended this comparison to the genomic level. Compared with the genome of the parent strain DSM 1731, two insertion sites, four deletion sites, and 67 single nucleotide variations (SNVs) are distributed throughout the genome of strain Rh8. Among the 67 SNVs, 16 SNVs are located in the predicted promoters and intergenic regions; while 29 SNVs are located in the coding sequence, affecting a total of 21 proteins involved in transport, cell structure, DNA replication, and protein translation. The remaining 22 SNVs are located in the ribosomal genes, affecting a total of 12 rRNA genes in different operons. Analysis of previous comparative proteomic data indicated that none of the differentially expressed proteins have mutations in its corresponding genes. Rchange Algorithms analysis indicated that the mutations occurred in the ribosomal genes might change the ribosome RNA thermodynamic characteristics, thus affect the translation strength of these proteins. Take together, the improved butanol tolerance of C. acetobutylicum strain Rh8 might be acquired through regulating the translational process to achieve different expression strength of genes involved in butanol tolerance.},
doi = {10.1016/J.BBRC.2014.07.052},
url = {https://www.osti.gov/biblio/22416701}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 450,
place = {United States},
year = {Fri Aug 08 00:00:00 EDT 2014},
month = {Fri Aug 08 00:00:00 EDT 2014}
}