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Title: Long-term low-dose α-particle enhanced the potential of malignant transformation in human bronchial epithelial cells through MAPK/Akt pathway

Abstract

Highlights: • Multi-exposures of 25 mGy α-ray enhanced cell proliferation, adhesion, and invasion. • MAPK/Akt but not JNK/P66 was positively correlated with cell invasive phenotypes. • LDR of α-irradiation triggers cell malignant transformation through MAPK/Akt. - Abstract: Since the wide usage of ionizing radiation, the cancer risk of low dose radiation (LDR) (<0.1 Gy) has become attractive for a long time. However, most results are derived from epidemiologic studies on atomic-bomb survivors and nuclear accidents surrounding population, and the molecular mechanism of this risk is elusive. To explore the potential of a long-term LDR-induced malignant transformation, human bronchial epithelial cells Beas-2B were fractionally irradiated with 0.025 Gy α-particles for 8 times in total and then further cultured for 1–2 months. It was found that the cell proliferation, the abilities of adhesion and invasion, and the protein expressions of p-ERK, p-Akt, especially p-P38 were not only increased in the multiply-irradiated cells but also in their offspring 1–2 months after the final exposure, indicating high potentiality of cell malignant transformation. On opposite, the expressions of p-JNK and p-P66 were diminished in the subcultures of irradiated cells and thus may play a role of negative regulation in canceration. When the cells were transferredmore » with p38 siRNA, the LDR-induced enhancements of cell adhesion and invasion were significantly reduced. These findings suggest that long-term LDR of α-particles could enhance the potential of malignant transformation incidence in human bronchial epithelial cells through MAPK/Akt pathway.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22416420
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 447; Journal Issue: 3; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADHESION; BIOLOGICAL RADIATION EFFECTS; CELL PROLIFERATION; HAZARDS; HUMAN POPULATIONS; IRRADIATION; NEOPLASMS; PHENOTYPE; PROGENY; PROTEINS; RADIATION ACCIDENTS; RADIATION DOSES

Citation Formats

Liu, Weili, Xiao, Linlin, Dong, Chen, He, Mingyuan, Pan, Yan, Xie, Yuexia, Tu, Wenzhi, Fu, Jiamei, and Shao, Chunlin. Long-term low-dose α-particle enhanced the potential of malignant transformation in human bronchial epithelial cells through MAPK/Akt pathway. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.03.159.
Liu, Weili, Xiao, Linlin, Dong, Chen, He, Mingyuan, Pan, Yan, Xie, Yuexia, Tu, Wenzhi, Fu, Jiamei, & Shao, Chunlin. Long-term low-dose α-particle enhanced the potential of malignant transformation in human bronchial epithelial cells through MAPK/Akt pathway. United States. https://doi.org/10.1016/J.BBRC.2014.03.159
Liu, Weili, Xiao, Linlin, Dong, Chen, He, Mingyuan, Pan, Yan, Xie, Yuexia, Tu, Wenzhi, Fu, Jiamei, and Shao, Chunlin. 2014. "Long-term low-dose α-particle enhanced the potential of malignant transformation in human bronchial epithelial cells through MAPK/Akt pathway". United States. https://doi.org/10.1016/J.BBRC.2014.03.159.
@article{osti_22416420,
title = {Long-term low-dose α-particle enhanced the potential of malignant transformation in human bronchial epithelial cells through MAPK/Akt pathway},
author = {Liu, Weili and Xiao, Linlin and Dong, Chen and He, Mingyuan and Pan, Yan and Xie, Yuexia and Tu, Wenzhi and Fu, Jiamei and Shao, Chunlin},
abstractNote = {Highlights: • Multi-exposures of 25 mGy α-ray enhanced cell proliferation, adhesion, and invasion. • MAPK/Akt but not JNK/P66 was positively correlated with cell invasive phenotypes. • LDR of α-irradiation triggers cell malignant transformation through MAPK/Akt. - Abstract: Since the wide usage of ionizing radiation, the cancer risk of low dose radiation (LDR) (<0.1 Gy) has become attractive for a long time. However, most results are derived from epidemiologic studies on atomic-bomb survivors and nuclear accidents surrounding population, and the molecular mechanism of this risk is elusive. To explore the potential of a long-term LDR-induced malignant transformation, human bronchial epithelial cells Beas-2B were fractionally irradiated with 0.025 Gy α-particles for 8 times in total and then further cultured for 1–2 months. It was found that the cell proliferation, the abilities of adhesion and invasion, and the protein expressions of p-ERK, p-Akt, especially p-P38 were not only increased in the multiply-irradiated cells but also in their offspring 1–2 months after the final exposure, indicating high potentiality of cell malignant transformation. On opposite, the expressions of p-JNK and p-P66 were diminished in the subcultures of irradiated cells and thus may play a role of negative regulation in canceration. When the cells were transferred with p38 siRNA, the LDR-induced enhancements of cell adhesion and invasion were significantly reduced. These findings suggest that long-term LDR of α-particles could enhance the potential of malignant transformation incidence in human bronchial epithelial cells through MAPK/Akt pathway.},
doi = {10.1016/J.BBRC.2014.03.159},
url = {https://www.osti.gov/biblio/22416420}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 447,
place = {United States},
year = {Fri May 09 00:00:00 EDT 2014},
month = {Fri May 09 00:00:00 EDT 2014}
}