miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression
Abstract
Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.
- Authors:
-
- Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 (China)
- Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 (China)
- Publication Date:
- OSTI Identifier:
- 22416263
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 444; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANIMAL CELLS; ANIMAL TISSUES; CELL PROLIFERATION; INHIBITION; METASTASES; OSTEOSARCOMAS; PHOSPHATASES; RNA
Citation Formats
Gao, Yong, Luo, Ling-hui, Li, Shuai, and Yang, Cao. miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression. United States: N. p., 2014.
Web. doi:10.1016/J.BBRC.2014.01.061.
Gao, Yong, Luo, Ling-hui, Li, Shuai, & Yang, Cao. miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression. United States. https://doi.org/10.1016/J.BBRC.2014.01.061
Gao, Yong, Luo, Ling-hui, Li, Shuai, and Yang, Cao. 2014.
"miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression". United States. https://doi.org/10.1016/J.BBRC.2014.01.061.
@article{osti_22416263,
title = {miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression},
author = {Gao, Yong and Luo, Ling-hui and Li, Shuai and Yang, Cao},
abstractNote = {Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.},
doi = {10.1016/J.BBRC.2014.01.061},
url = {https://www.osti.gov/biblio/22416263},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 444,
place = {United States},
year = {Fri Feb 07 00:00:00 EST 2014},
month = {Fri Feb 07 00:00:00 EST 2014}
}