Plasmon resonance enhanced mechanical detection of ligand binding

Journal Article · Mon Jan 05 00:00:00 EST 2015 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.4905550· OSTI ID:22395679

Ariyaratne, Amila; Zocchi, Giovanni ^[1]

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1. Department of Physics and Astronomy, University of California Los Angeles, Los Angeles, California 90095 (United States)

Small molecule binding to the active site of enzymes typically modifies the mechanical stiffness of the enzyme. We exploit this effect, in a setup which combines nano-mechanics and surface plasmon resonance (SPR) enhanced optics, for the label free detection of ligand binding to an enzyme. The large dynamic range of the signal allows to easily obtain binding curves for small ligands, in contrast to traditional SPR methods which rely on small changes in index of refraction. Enzyme mechanics, assessed by nano-rheology, thus emerges as an alternative to electronic and spin resonances, assessed by traditional spectroscopies, for detecting ligand binding.

• OSTI ID: 22395679
• Journal Information: Applied Physics Letters, Vol. 106, Issue 1; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
DIAGRAMS
ENZYMES
FLEXIBILITY
LIGANDS
MOLECULES
NANOSTRUCTURES
OPTICS
PLASMONS
REFRACTIVE INDEX
RESONANCE
RHEOLOGY
SPECTROSCOPY
SPIN
SURFACES