Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2
- Department of Biology, Illinois Institute of Technology, Chicago, IL 60616 (United States)
The crystallization of the brazil nut allergen Ber e 2 is reported. Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.
- OSTI ID:
- 22360434
- Journal Information:
- Acta Crystallographica. Section F, Vol. 63, Issue Pt 11; Other Information: PMCID: PMC2339761; PMID: 18007055; PUBLISHER-ID: rp5009; OAI: oai:pubmedcentral.nih.gov:2339761; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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