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Title: Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase

Abstract

Preliminary X-ray diffraction studies of apo maize aldose reductase at 2.0 Å resolution are reported. Maize aldose reductase (AR) is a member of the aldo-keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 47.2, b = 54.5, c = 100.6 Å and one molecule in the asymmetric unit. Synchrotron X-ray diffraction data were collected and a final resolution limit of 2.0 Å was obtained after data reduction. Phasing was carried out by an automated molecular-replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.

Authors:
 [1];  [2]; ;  [1];  [3];  [4];  [1]
  1. Laboratório de Biologia Estrutural, Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas-SP (Brazil)
  2. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas-SP (Brazil)
  3. Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas-SP (Brazil)
  4. Laboratório de Biologia Molecular, Centro Infantil Boldrini, Campinas-SP (Brazil)
Publication Date:
OSTI Identifier:
22360424
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 63; Journal Issue: Pt 11; Other Information: PMCID: PMC2339750; PMID: 18007059; PUBLISHER-ID: fw5152; OAI: oai:pubmedcentral.nih.gov:2339750; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CONVERSION; CRYSTALLIZATION; GLUCOSE; MOLECULES; REDUCTION; RESOLUTION; SPACE GROUPS; SYNCHROTRONS; VISIBLE RADIATION; X-RAY DIFFRACTION

Citation Formats

Kiyota, Eduardo, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas-SP, Sousa, Sylvia Morais de, Santos, Marcelo Leite dos, Costa Lima, Aline da, Menossi, Marcelo, Yunes, José Andrés, and Aparicio, Ricardo. Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107052670.
Kiyota, Eduardo, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas-SP, Sousa, Sylvia Morais de, Santos, Marcelo Leite dos, Costa Lima, Aline da, Menossi, Marcelo, Yunes, José Andrés, & Aparicio, Ricardo. Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase. United Kingdom. https://doi.org/10.1107/S1744309107052670
Kiyota, Eduardo, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas-SP, Sousa, Sylvia Morais de, Santos, Marcelo Leite dos, Costa Lima, Aline da, Menossi, Marcelo, Yunes, José Andrés, and Aparicio, Ricardo. 2007. "Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase". United Kingdom. https://doi.org/10.1107/S1744309107052670.
@article{osti_22360424,
title = {Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase},
author = {Kiyota, Eduardo and Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas-SP and Sousa, Sylvia Morais de and Santos, Marcelo Leite dos and Costa Lima, Aline da and Menossi, Marcelo and Yunes, José Andrés and Aparicio, Ricardo},
abstractNote = {Preliminary X-ray diffraction studies of apo maize aldose reductase at 2.0 Å resolution are reported. Maize aldose reductase (AR) is a member of the aldo-keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 47.2, b = 54.5, c = 100.6 Å and one molecule in the asymmetric unit. Synchrotron X-ray diffraction data were collected and a final resolution limit of 2.0 Å was obtained after data reduction. Phasing was carried out by an automated molecular-replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.},
doi = {10.1107/S1744309107052670},
url = {https://www.osti.gov/biblio/22360424}, journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 11,
volume = 63,
place = {United Kingdom},
year = {Thu Nov 01 00:00:00 EDT 2007},
month = {Thu Nov 01 00:00:00 EDT 2007}
}