Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of XC847, a 3′-5′ oligoribonuclease from Xanthomonas campestris
- Department of Life Science, National Central University, Jung-Li 300, Taoyuan,Taiwan (China)
- Institute of Biochemistry, National Chung-Hsing University, Taichung 40227,Taiwan (China)
- Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei,Taiwan (China)
- Core Facility for Protein Crystallography, Academia Sinica, Nankang, Taipei,Taiwan (China)
- National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310 (United States)
- Department of Life Science, National Tsing Hua University, Hsin-Chu,Taiwan (China)
A DEDDh-type 3′-5′ oligoribonuclease from the plant pathogen X. campestris pv. campestris has been overexpressed in E. coli, purified and crystallized. The crystals diffracted to 2.1 Å with good quality. Oligoribonucleases are essential components of RNA and DNA metabolism and close homologues of genes encoding them are found not only in prokaryotes but also in a wide range of eukaryotes, including yeast and humans. Inactivation of the oligoribonuclease gene (orn) can result in cellular lethality. Despite their important biological function, they have been studied little from a structural point of view. In this report, the cloning, expression, crystallization and preliminary X-ray analysis of XC847, a DEDDh-type 3′-5′ oligoribonuclease from the plant pathogen Xanthomonas campestris pv. campestris, a Gram-negative bacterium causing major worldwide disease of cruciferous crops, is described. The XC847 crystals diffracted to a resolution of at least 2.1 Å. They are tetragonal and belong to space group P4{sub 3}2{sub 1}2, with unit-cell parameters a = b = 67.5, c = 89.8 Å. One molecule is present per asymmetric unit.
- OSTI ID:
- 22356231
- Journal Information:
- Acta Crystallographica. Section F, Vol. 61, Issue Pt 10; Other Information: PMCID: PMC1991326; PMID: 16511191; PUBLISHER-ID: vr5046; OAI: oai:pubmedcentral.nih.gov:1991326; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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