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Title: Crystallization of a protein using dehydration without a precipitant

Abstract

Crystals grown by simple dehydration from a solution of hypoxic response protein I (HRPI) from M. tuberculosis diffract to a maximum resolution of 2.1 Å Hypoxic response protein I (HRPI) is a protein of unknown biochemical function whose expression is very strongly upregulated in response to oxygen depletion in Mycobacterium tuberculosis. Crystals have been grown from a solution of full-length HRPI by the unusual method of dehydration without the use of precipitants. The crystals produced diffract to a maximum resolution of 2.1 Å and belong to space group P4{sub 1}2{sub 1}2 (or P4{sub 3}2{sub 1}2), with unit-cell parameters a = b = 79.18, c = 37.34 Å.

Authors:
;  [1]
  1. School of Biological Sciences and Centre for Molecular Biodiscovery, University of Auckland, Private Bag 92-019, Auckland 1003 (New Zealand)
Publication Date:
OSTI Identifier:
22356029
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 61; Journal Issue: Pt 6; Other Information: PMCID: PMC1952335; PMID: 16511097; PUBLISHER-ID: gx5055; OAI: oai:pubmedcentral.nih.gov:1952335; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DEHYDRATION; LENGTH; MATHEMATICAL SOLUTIONS; OXYGEN; PROTEINS; RESOLUTION; SOLUTIONS; SPACE GROUPS

Citation Formats

Sharpe, Miriam L., E-mail: m.sharpe@auckland.ac.nz, Baker, Edward N., and Lott, J. Shaun. Crystallization of a protein using dehydration without a precipitant. United Kingdom: N. p., 2005. Web. doi:10.1107/S1744309105014235.
Sharpe, Miriam L., E-mail: m.sharpe@auckland.ac.nz, Baker, Edward N., & Lott, J. Shaun. Crystallization of a protein using dehydration without a precipitant. United Kingdom. https://doi.org/10.1107/S1744309105014235
Sharpe, Miriam L., E-mail: m.sharpe@auckland.ac.nz, Baker, Edward N., and Lott, J. Shaun. 2005. "Crystallization of a protein using dehydration without a precipitant". United Kingdom. https://doi.org/10.1107/S1744309105014235.
@article{osti_22356029,
title = {Crystallization of a protein using dehydration without a precipitant},
author = {Sharpe, Miriam L., E-mail: m.sharpe@auckland.ac.nz and Baker, Edward N. and Lott, J. Shaun},
abstractNote = {Crystals grown by simple dehydration from a solution of hypoxic response protein I (HRPI) from M. tuberculosis diffract to a maximum resolution of 2.1 Å Hypoxic response protein I (HRPI) is a protein of unknown biochemical function whose expression is very strongly upregulated in response to oxygen depletion in Mycobacterium tuberculosis. Crystals have been grown from a solution of full-length HRPI by the unusual method of dehydration without the use of precipitants. The crystals produced diffract to a maximum resolution of 2.1 Å and belong to space group P4{sub 1}2{sub 1}2 (or P4{sub 3}2{sub 1}2), with unit-cell parameters a = b = 79.18, c = 37.34 Å.},
doi = {10.1107/S1744309105014235},
url = {https://www.osti.gov/biblio/22356029}, journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 6,
volume = 61,
place = {United Kingdom},
year = {Wed Jun 01 00:00:00 EDT 2005},
month = {Wed Jun 01 00:00:00 EDT 2005}
}