Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA
- Department of Chemistry, University of Arizona, Tucson, AZ 85721 (United States)
- Albert Einstein College of Medicine, New York, NY (United States)
- Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037 (United States)
- Department of Pharmacology and Toxicology, University of Arizona, Tucson, AZ 85721 (United States)
Preliminary data analysis of crystals of a designed six-finger zinc-finger protein bound to DNA is presented. The cell likely contains two protein–DNA complexes and diffracts to 2.95 Å. Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 Å, α = 100.87, β = 96.22, γ = 106.33°. There are most likely to be two protein–DNA complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.95 Å at 100 K, with an R{sub merge} of 9.3%.
- OSTI ID:
- 22356026
- Journal Information:
- Acta Crystallographica. Section F, Vol. 61, Issue Pt 6; Other Information: PMCID: PMC1952332; PMID: 16511099; PUBLISHER-ID: za5100; OAI: oai:pubmedcentral.nih.gov:1952332; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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Crystallization And Preliminary X-Ray Crystallographic Analysis of Aart, a Designed Six-Finger Zinc-Finger Peptide, Bound to Dna