Split green fluorescent protein as a modular binding partner for protein crystallization

Nguyen, Hau B.; Hung, Li-Wei; Yeates, Todd O.

doi:10.1107/S0907444913024608

Title: Split green fluorescent protein as a modular binding partner for protein crystallization

Journal Article · Sun Dec 01 00:00:00 EST 2013 · Acta Crystallographica. Section D: Biological Crystallography

DOI:https://doi.org/10.1107/S0907444913024608· OSTI ID:22347805

Nguyen, Hau B. ^[1]; Hung, Li-Wei ^[2]; Yeates, Todd O. ^[3]

Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States)
Los Alamos National Laboratory, MS D454, Los Alamos, NM 87545 (United States)
University of California, PO Box 951569, Los Angeles, CA 90095 (United States)

A strategy using a new split green fluorescent protein (GFP) as a modular binding partner to form stable protein complexes with a target protein is presented. The modular split GFP may open the way to rapidly creating crystallization variants. A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10–11) hairpin in complex with GFP(1–9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10–11) hairpin with a variety of GFP(1–9) mutants engineered for favorable crystallization.

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OSTI ID:: 22347805

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography, Vol. 69, Issue Pt 12; Other Information: PMCID: PMC3852656; PMID: 24311592; PUBLISHER-ID: dz5297; OAI: oai:pubmedcentral.nih.gov:3852656; Copyright (c) Nguyen et al. 2013; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449

Country of Publication:: Denmark

Language:: English

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