In cellulo structure determination of a novel cypovirus polyhedrin
- Diamond Light Source Ltd, Harwell Oxford, Didcot OX11 0DE (United Kingdom)
- University of Oxford, Oxford OX3 7BN (United Kingdom)
The crystal structure of a previously unsolved type of cypovirus polyhedrin has been determined from data collected directly from frozen live insect cells. This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.
- OSTI ID:
- 22347799
- Journal Information:
- Acta Crystallographica. Section D: Biological Crystallography, Vol. 70, Issue Pt 5; Other Information: PMCID: PMC4014125; PMID: 24816111; PUBLISHER-ID: wa5065; OAI: oai:pubmedcentral.nih.gov:4014125; Copyright (c) Axford et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449
- Country of Publication:
- Denmark
- Language:
- English
Similar Records
Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data
Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs