Copper oxide assisted cysteine hierarchical structures for immunosensor application

Journal Article · Mon Sep 08 00:00:00 EDT 2014 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.4895579· OSTI ID:22310856

Pandey, Chandra Mouli ^[1]; Sumana, Gajjala ^[1]; Tiwari, Ida ^[2]

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1. Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi 110012 (India)
2. Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005 (India)

The present work describes the promising electrochemical immunosensing strategy based on copper (II) assisted hierarchical cysteine structures (CuCys) varying from star to flower like morphology. The CuCys having average size of 10 μm have been synthesised using L-Cysteine as initial precursor in presence of copper oxide under environmentally friendly conditions in aqueous medium. To delineate the synthesis mechanism, detailed structural investigations have been carried out using characterization techniques such as X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The electrochemical behaviour of self-assembled CuCys on gold electrode shows surface controlled electrode reaction with an apparent electron transfer rate constant of 3.38 × 10{sup −4 }cm s{sup −1}. This innovative platform has been utilized to fabricate an immunosensor by covalently immobilizing monoclonal antibodies specific for Escherichia coli O157:H7 (E. coli). Under the optimal conditions, the fabricated immunosensor is found to be sensitive and specific for the detection of E. coli with a detection limit of 10 cfu/ml.

• OSTI ID: 22310856
• Journal Information: Applied Physics Letters, Vol. 105, Issue 10; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
BIOLOGICAL EFFECTS
COPPER
COPPER OXIDES
COVALENCE
CYSTEINE
DETECTION
ELECTROCHEMISTRY
ELECTRODES
ELECTRON TRANSFER
ESCHERICHIA COLI
FOURIER TRANSFORM SPECTROMETERS
GOLD
MONOCLONAL ANTIBODIES
SENSITIVITY
SENSORS
SURFACES
SYNTHESIS
TRANSMISSION ELECTRON MICROSCOPY
X-RAY DIFFRACTION