Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines
Abstract
Scaffold/matrix attachment regions (S/MARs) are potential element that can be integrated into expression vector to increase expression of recombinant protein. Many studies on S/MAR have been done but none has revealed the distribution of S/MAR in a genome. In this study, we have isolated S/MAR sequences from HEK293 and Chinese hamster ovary cell lines (CHO DG44) using two different methods utilizing 2 M NaCl and lithium-3,5-diiodosalicylate (LIS). The isolated S/MARs were sequenced using Next Generation Sequencing (NGS) platform. Based on reference mapping analysis against human genome database, a total of 8,994,856 and 8,412,672 contigs of S/MAR sequences were retrieved from 2M NaCl and LIS extraction of HEK293 respectively. On the other hand, reference mapping analysis of S/MAR derived from CHO DG44 against our own CHO DG44 database have generated a total of 7,204,348 and 4,672,913 contigs from 2 M NaCl and LIS extraction method respectively.
- Authors:
-
- School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia)
- Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia)
- Publication Date:
- OSTI Identifier:
- 22308343
- Resource Type:
- Journal Article
- Journal Name:
- AIP Conference Proceedings
- Additional Journal Information:
- Journal Volume: 1614; Journal Issue: 1; Conference: 2014 UKM FST postgraduate colloquium, Selangor (Malaysia), 9-11 Apr 2014; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; CHO CELLS; DISTRIBUTION; EXTRACTION; HUMAN POPULATIONS; MAPPING; MATRICES; PROTEINS; SODIUM CHLORIDES; VECTORS
Citation Formats
Pilus, Nur Shazwani Mohd, Ahmad, Azrin, Yusof, Nurul Yuziana Mohd, and Johari, Norazfa. Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines. United States: N. p., 2014.
Web. doi:10.1063/1.4895256.
Pilus, Nur Shazwani Mohd, Ahmad, Azrin, Yusof, Nurul Yuziana Mohd, & Johari, Norazfa. Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines. United States. https://doi.org/10.1063/1.4895256
Pilus, Nur Shazwani Mohd, Ahmad, Azrin, Yusof, Nurul Yuziana Mohd, and Johari, Norazfa. 2014.
"Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines". United States. https://doi.org/10.1063/1.4895256.
@article{osti_22308343,
title = {Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines},
author = {Pilus, Nur Shazwani Mohd and Ahmad, Azrin and Yusof, Nurul Yuziana Mohd and Johari, Norazfa},
abstractNote = {Scaffold/matrix attachment regions (S/MARs) are potential element that can be integrated into expression vector to increase expression of recombinant protein. Many studies on S/MAR have been done but none has revealed the distribution of S/MAR in a genome. In this study, we have isolated S/MAR sequences from HEK293 and Chinese hamster ovary cell lines (CHO DG44) using two different methods utilizing 2 M NaCl and lithium-3,5-diiodosalicylate (LIS). The isolated S/MARs were sequenced using Next Generation Sequencing (NGS) platform. Based on reference mapping analysis against human genome database, a total of 8,994,856 and 8,412,672 contigs of S/MAR sequences were retrieved from 2M NaCl and LIS extraction of HEK293 respectively. On the other hand, reference mapping analysis of S/MAR derived from CHO DG44 against our own CHO DG44 database have generated a total of 7,204,348 and 4,672,913 contigs from 2 M NaCl and LIS extraction method respectively.},
doi = {10.1063/1.4895256},
url = {https://www.osti.gov/biblio/22308343},
journal = {AIP Conference Proceedings},
issn = {0094-243X},
number = 1,
volume = 1614,
place = {United States},
year = {Wed Sep 03 00:00:00 EDT 2014},
month = {Wed Sep 03 00:00:00 EDT 2014}
}