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Title: A flavone derivative from Sesbania sesban leaves and its cytotoxicity against murine leukemia P-388 cells

Abstract

Sesbania sesban, locally named as Jayanti, is one of Indonesia plants belonging to Fabaceae family. This species is traditionally used by Indonesian people to cure digestive disorders, fever, or headache. Jayanti can grow well in tropical to subtropical region, such as in Asia and Africa. Based on literature, qualitative analysis of the methanol extract of leaves of S. sesban showed that it contained flavonoids, alkaloids, saponins and glycosides. In addition, the activity assay of extracts of different tissues of this species showed antitumor, antimalarial, and antidiabetic activityies (leaves and seed extracts), antioxidants (flower extract), and analgesic (wood extract). Though the extracts of S. sesban parts showed interesting activities, chemical study of those extracts have not been widely reported. Therefore, the objective of this research was to isolate the secondary metabolites from methanol extract of leaves of S. sesban and to determine their cytotoxicity against murine leukemia P-388 cells. One compound has been obtained and identified as 3-hydroxy-4',7-dimethoxyflavone (1), a new isolated compound from nature. This compound was obtained through separation of methanol extract using various chromatographic techniques, such as vacuum liquid chromatography and radial chromatography. The structure elucidation of isolated compound was based on 1D NMR ({sup 1}H-NMR and {supmore » 13}C-NMR) and 2D NMR (HMBC). The cytotoxicity of methanol extract and compound 1 against murine leukemia P-388 cells examined through MTT assay showed IC{sub 50} value of 60.04 μg/mL and 5.40 μg/mL, respectively.« less

Authors:
;
Publication Date:
OSTI Identifier:
22265985
Resource Type:
Journal Article
Journal Name:
AIP Conference Proceedings
Additional Journal Information:
Journal Volume: 1589; Journal Issue: 1; Conference: ICMNS 2012: 4. international conference on mathematics and natural sciences: Science for health, food and sustainable energy, Bandung (Indonesia), 8-9 Nov 2012; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0094-243X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALKALOIDS; ANALGESICS; ANTIOXIDANTS; FLAVONOIDS; LEAVES; LEUKEMIA; LIQUID COLUMN CHROMATOGRAPHY; METABOLITES; METHANOL; NUCLEAR MAGNETIC RESONANCE; PLANT TISSUES; PLANTS; SAPONINS; TOXICITY

Citation Formats

Dianhar, Hanhan, Syah, Yana Maolana, E-mail: liadewi@chem.itb.ac.id, Mujahidin, Didin, Hakim, Euis Holisotan, E-mail: liadewi@chem.itb.ac.id, and Juliawaty, Lia Dewi, E-mail: liadewi@chem.itb.ac.id. A flavone derivative from Sesbania sesban leaves and its cytotoxicity against murine leukemia P-388 cells. United States: N. p., 2014. Web. doi:10.1063/1.4868831.
Dianhar, Hanhan, Syah, Yana Maolana, E-mail: liadewi@chem.itb.ac.id, Mujahidin, Didin, Hakim, Euis Holisotan, E-mail: liadewi@chem.itb.ac.id, & Juliawaty, Lia Dewi, E-mail: liadewi@chem.itb.ac.id. A flavone derivative from Sesbania sesban leaves and its cytotoxicity against murine leukemia P-388 cells. United States. https://doi.org/10.1063/1.4868831
Dianhar, Hanhan, Syah, Yana Maolana, E-mail: liadewi@chem.itb.ac.id, Mujahidin, Didin, Hakim, Euis Holisotan, E-mail: liadewi@chem.itb.ac.id, and Juliawaty, Lia Dewi, E-mail: liadewi@chem.itb.ac.id. 2014. "A flavone derivative from Sesbania sesban leaves and its cytotoxicity against murine leukemia P-388 cells". United States. https://doi.org/10.1063/1.4868831.
@article{osti_22265985,
title = {A flavone derivative from Sesbania sesban leaves and its cytotoxicity against murine leukemia P-388 cells},
author = {Dianhar, Hanhan and Syah, Yana Maolana, E-mail: liadewi@chem.itb.ac.id and Mujahidin, Didin and Hakim, Euis Holisotan, E-mail: liadewi@chem.itb.ac.id and Juliawaty, Lia Dewi, E-mail: liadewi@chem.itb.ac.id},
abstractNote = {Sesbania sesban, locally named as Jayanti, is one of Indonesia plants belonging to Fabaceae family. This species is traditionally used by Indonesian people to cure digestive disorders, fever, or headache. Jayanti can grow well in tropical to subtropical region, such as in Asia and Africa. Based on literature, qualitative analysis of the methanol extract of leaves of S. sesban showed that it contained flavonoids, alkaloids, saponins and glycosides. In addition, the activity assay of extracts of different tissues of this species showed antitumor, antimalarial, and antidiabetic activityies (leaves and seed extracts), antioxidants (flower extract), and analgesic (wood extract). Though the extracts of S. sesban parts showed interesting activities, chemical study of those extracts have not been widely reported. Therefore, the objective of this research was to isolate the secondary metabolites from methanol extract of leaves of S. sesban and to determine their cytotoxicity against murine leukemia P-388 cells. One compound has been obtained and identified as 3-hydroxy-4',7-dimethoxyflavone (1), a new isolated compound from nature. This compound was obtained through separation of methanol extract using various chromatographic techniques, such as vacuum liquid chromatography and radial chromatography. The structure elucidation of isolated compound was based on 1D NMR ({sup 1}H-NMR and {sup 13}C-NMR) and 2D NMR (HMBC). The cytotoxicity of methanol extract and compound 1 against murine leukemia P-388 cells examined through MTT assay showed IC{sub 50} value of 60.04 μg/mL and 5.40 μg/mL, respectively.},
doi = {10.1063/1.4868831},
url = {https://www.osti.gov/biblio/22265985}, journal = {AIP Conference Proceedings},
issn = {0094-243X},
number = 1,
volume = 1589,
place = {United States},
year = {Mon Mar 24 00:00:00 EDT 2014},
month = {Mon Mar 24 00:00:00 EDT 2014}
}