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Title: A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions

Abstract

We present a novel facility for micro-irradiation of living targets with ions from a 1.7 MV tandem accelerator. We show results using 1 MeV protons and 2 MeV He{sup 2+}. In contrast to common micro-irradiation facilities, which use electromagnetic or electrostatic focusing and specially designed vacuum windows, we employ a tapered glass capillary with a thin end window, made from polystyrene with a thickness of 1–2 μm, for ion focusing and extraction. The capillary is connected to a beamline tilted vertically by 45°, which allows for easy immersion of the extracted ions into liquid environment within a standard cell culture dish. An inverted microscope is used for simultaneously observing the samples as well as the capillary tip, while a stage-top incubator provides an appropriate environment for the samples. Furthermore, our setup allows to target volumes in cells within a μm{sup 3} resolution, while monitoring the target in real time during and after irradiation.

Authors:
; ; ; ; ; ;  [1]; ;  [2]
  1. Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama (Japan)
  2. Cellular Dynamics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama (Japan)
Publication Date:
OSTI Identifier:
22251198
Resource Type:
Journal Article
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 85; Journal Issue: 1; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0034-6748
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 60 APPLIED LIFE SCIENCES; CAPILLARIES; CELL CULTURES; EXTRACTION; FOCUSING; HELIUM IONS; IRRADIATION; IRRADIATION PLANTS; MICROSCOPES; POLYSTYRENE; TANDEM ELECTROSTATIC ACCELERATORS; THICKNESS

Citation Formats

Mäckel, V., E-mail: maeckel@riken.jp, Meissl, W., Ikeda, T., Meissl, E., Kobayashi, T., Kojima, T. M., Ogiwara, K., Yamazaki, Y., Clever, M., and Imamoto, N. A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions. United States: N. p., 2014. Web. doi:10.1063/1.4859499.
Mäckel, V., E-mail: maeckel@riken.jp, Meissl, W., Ikeda, T., Meissl, E., Kobayashi, T., Kojima, T. M., Ogiwara, K., Yamazaki, Y., Clever, M., & Imamoto, N. A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions. United States. https://doi.org/10.1063/1.4859499
Mäckel, V., E-mail: maeckel@riken.jp, Meissl, W., Ikeda, T., Meissl, E., Kobayashi, T., Kojima, T. M., Ogiwara, K., Yamazaki, Y., Clever, M., and Imamoto, N. 2014. "A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions". United States. https://doi.org/10.1063/1.4859499.
@article{osti_22251198,
title = {A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions},
author = {Mäckel, V., E-mail: maeckel@riken.jp and Meissl, W. and Ikeda, T. and Meissl, E. and Kobayashi, T. and Kojima, T. M. and Ogiwara, K. and Yamazaki, Y. and Clever, M. and Imamoto, N.},
abstractNote = {We present a novel facility for micro-irradiation of living targets with ions from a 1.7 MV tandem accelerator. We show results using 1 MeV protons and 2 MeV He{sup 2+}. In contrast to common micro-irradiation facilities, which use electromagnetic or electrostatic focusing and specially designed vacuum windows, we employ a tapered glass capillary with a thin end window, made from polystyrene with a thickness of 1–2 μm, for ion focusing and extraction. The capillary is connected to a beamline tilted vertically by 45°, which allows for easy immersion of the extracted ions into liquid environment within a standard cell culture dish. An inverted microscope is used for simultaneously observing the samples as well as the capillary tip, while a stage-top incubator provides an appropriate environment for the samples. Furthermore, our setup allows to target volumes in cells within a μm{sup 3} resolution, while monitoring the target in real time during and after irradiation.},
doi = {10.1063/1.4859499},
url = {https://www.osti.gov/biblio/22251198}, journal = {Review of Scientific Instruments},
issn = {0034-6748},
number = 1,
volume = 85,
place = {United States},
year = {Wed Jan 15 00:00:00 EST 2014},
month = {Wed Jan 15 00:00:00 EST 2014}
}