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Title: CD8{sup +}CD25{sup +} T cells reduce atherosclerosis in apoE(−/−) mice

Abstract

Highlights: •The role of a sub-population of CD8{sup +} T cells with suppressor functions was investigated in atherosclerosis. •CD8{sup +}CD25{sup +} T cells from adult apoE(−/−) mice had phenotype characteristics of T suppressor cells. •These CD8{sup +}CD25{sup +} T cells reduced CD4{sup +} T cell proliferation and CD8{sup +} cytotoxic activity in vitro. •Adoptive transfer of CD8{sup +}CD25{sup +} T cells significantly reduced atherosclerosis. •CD8{sup +}CD25{sup +} T cells have a suppressive function in atherosclerosis. -- Abstract: Background: It is increasingly evident that CD8{sup +} T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8{sup +}CD25{sup +} T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8{sup +}CD25{sup +} T cells in experimental atherosclerosis were investigated in this study. Methods and results: CD8{sup +}CD25{sup +} T cells were observed in atherosclerotic plaques of apoE(−/−) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8{sup +}CD25{sup +} T cells from apoE(−/−) mice. Depletion of CD8{sup +}CD25{sup +} from total CD8{supmore » +} T cells rendered higher cytolytic activity of the remaining CD8{sup +}CD25{sup −} T cells. Adoptive transfer of CD8{sup +}CD25{sup +} T cells into apoE(−/−) mice suppressed the proliferation of splenic CD4{sup +} T cells and significantly reduced atherosclerosis in recipient mice. Conclusions: Our study has identified an athero-protective role for CD8{sup +}CD25{sup +} T cells in experimental atherosclerosis.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22242278
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 443; Journal Issue: 3; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARTERIOSCLEROSIS; CELL PROLIFERATION; EVALUATION; IN VITRO; MICE; PHENOTYPE

Citation Formats

Zhou, Jianchang, Dimayuga, Paul C., Zhao, Xiaoning, Yano, Juliana, Lio, Wai Man, Trinidad, Portia, Honjo, Tomoyuki, Cercek, Bojan, Shah, Prediman K., and Chyu, Kuang-Yuh. CD8{sup +}CD25{sup +} T cells reduce atherosclerosis in apoE(−/−) mice. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2013.12.057.
Zhou, Jianchang, Dimayuga, Paul C., Zhao, Xiaoning, Yano, Juliana, Lio, Wai Man, Trinidad, Portia, Honjo, Tomoyuki, Cercek, Bojan, Shah, Prediman K., & Chyu, Kuang-Yuh. CD8{sup +}CD25{sup +} T cells reduce atherosclerosis in apoE(−/−) mice. United States. https://doi.org/10.1016/J.BBRC.2013.12.057
Zhou, Jianchang, Dimayuga, Paul C., Zhao, Xiaoning, Yano, Juliana, Lio, Wai Man, Trinidad, Portia, Honjo, Tomoyuki, Cercek, Bojan, Shah, Prediman K., and Chyu, Kuang-Yuh. 2014. "CD8{sup +}CD25{sup +} T cells reduce atherosclerosis in apoE(−/−) mice". United States. https://doi.org/10.1016/J.BBRC.2013.12.057.
@article{osti_22242278,
title = {CD8{sup +}CD25{sup +} T cells reduce atherosclerosis in apoE(−/−) mice},
author = {Zhou, Jianchang and Dimayuga, Paul C. and Zhao, Xiaoning and Yano, Juliana and Lio, Wai Man and Trinidad, Portia and Honjo, Tomoyuki and Cercek, Bojan and Shah, Prediman K. and Chyu, Kuang-Yuh},
abstractNote = {Highlights: •The role of a sub-population of CD8{sup +} T cells with suppressor functions was investigated in atherosclerosis. •CD8{sup +}CD25{sup +} T cells from adult apoE(−/−) mice had phenotype characteristics of T suppressor cells. •These CD8{sup +}CD25{sup +} T cells reduced CD4{sup +} T cell proliferation and CD8{sup +} cytotoxic activity in vitro. •Adoptive transfer of CD8{sup +}CD25{sup +} T cells significantly reduced atherosclerosis. •CD8{sup +}CD25{sup +} T cells have a suppressive function in atherosclerosis. -- Abstract: Background: It is increasingly evident that CD8{sup +} T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8{sup +}CD25{sup +} T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8{sup +}CD25{sup +} T cells in experimental atherosclerosis were investigated in this study. Methods and results: CD8{sup +}CD25{sup +} T cells were observed in atherosclerotic plaques of apoE(−/−) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8{sup +}CD25{sup +} T cells from apoE(−/−) mice. Depletion of CD8{sup +}CD25{sup +} from total CD8{sup +} T cells rendered higher cytolytic activity of the remaining CD8{sup +}CD25{sup −} T cells. Adoptive transfer of CD8{sup +}CD25{sup +} T cells into apoE(−/−) mice suppressed the proliferation of splenic CD4{sup +} T cells and significantly reduced atherosclerosis in recipient mice. Conclusions: Our study has identified an athero-protective role for CD8{sup +}CD25{sup +} T cells in experimental atherosclerosis.},
doi = {10.1016/J.BBRC.2013.12.057},
url = {https://www.osti.gov/biblio/22242278}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 443,
place = {United States},
year = {Fri Jan 17 00:00:00 EST 2014},
month = {Fri Jan 17 00:00:00 EST 2014}
}