Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis

Shrestha, Chandan; Ito, Takashi; Kawahara, Ko-ichi; Shrestha, Binita; Yamakuchi, Munekazu; Hashiguchi, Teruto; Maruyama, Ikuro

doi:10.1016/J.BBRC.2013.06.117

Title: Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis

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Abstract

Highlights: •High-fat diet feeding and palmitate induces the release of nuclear protein histone H3. •ROS production and JNK signaling mediates the release of histone H3. •Extracellular histones induces proinflammatory and procoagulant response. -- Abstract: Chronic low-grade inflammation is a key contributor to high-fat diet (HFD)-related diseases, such as type 2 diabetes, non-alcoholic steatohepatitis, and atherosclerosis. The inflammation is characterized by infiltration of inflammatory cells, particularly macrophages, into obese adipose tissue. However, the molecular mechanisms by which a HFD induces low-grade inflammation are poorly understood. Here, we show that histone H3, a major protein component of chromatin, is released into the extracellular space when mice are fed a HFD or macrophages are stimulated with the saturated fatty acid palmitate. In a murine macrophage cell line, RAW 264.7, palmitate activated reactive oxygen species (ROS) production and JNK signaling. Inhibitors of these pathways dampened palmitate-induced histone H3 release, suggesting that the extracellular release of histone H3 was mediated, in part, through ROS and JNK signaling. Extracellular histone activated endothelial cells toexpress the adhesion molecules ICAM-1 and VCAM-1 and the procoagulant molecule tissue factor, which are known to contribute to inflammatory cell recruitment and thrombosis. These results suggest the possible contribution of extracellular histonemore »« less

Authors:

Shrestha, Chandan ^[1]; Ito, Takashi ^[1]; Kawahara, Ko-ichi ^[2]; Shrestha, Binita; Yamakuchi, Munekazu; Hashiguchi, Teruto ^[3]; Maruyama, Ikuro ^[1]

Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan)
Department of Biomedical Engineering, Osaka Institute of Technology, Osaka (Japan)
Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan)

Publication Date:: Fri Aug 09 00:00:00 EDT 2013

OSTI Identifier:: 22239695

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 437; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; ARTERIOSCLEROSIS; CARBOXYLIC ACIDS; CHROMATIN; DIET; EXTRACELLULAR SPACE; FATS; FEEDING; HISTONES; INFLAMMATION; MACROPHAGES; MICE; PATHOGENESIS; THROMBOSIS

Citation Formats


                    Shrestha, Chandan, Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Ito, Takashi, Kawahara, Ko-ichi, Shrestha, Binita, Yamakuchi, Munekazu, Hashiguchi, Teruto, and Maruyama, Ikuro. Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis.  United States: N. p., 2013. 
        Web.  doi:10.1016/J.BBRC.2013.06.117.

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                    Shrestha, Chandan, Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Ito, Takashi, Kawahara, Ko-ichi, Shrestha, Binita, Yamakuchi, Munekazu, Hashiguchi, Teruto, & Maruyama, Ikuro. Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis.  United States.  https://doi.org/10.1016/J.BBRC.2013.06.117

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                    Shrestha, Chandan, Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Ito, Takashi, Kawahara, Ko-ichi, Shrestha, Binita, Yamakuchi, Munekazu, Hashiguchi, Teruto, and Maruyama, Ikuro. 2013.  
        "Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis".  United States.  https://doi.org/10.1016/J.BBRC.2013.06.117.

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@article{osti_22239695,

  title        = {Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis},

  author       = {Shrestha, Chandan and Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima and Ito, Takashi and Kawahara, Ko-ichi and Shrestha, Binita and Yamakuchi, Munekazu and Hashiguchi, Teruto and Maruyama, Ikuro},

  abstractNote = {Highlights: •High-fat diet feeding and palmitate induces the release of nuclear protein histone H3. •ROS production and JNK signaling mediates the release of histone H3. •Extracellular histones induces proinflammatory and procoagulant response. -- Abstract: Chronic low-grade inflammation is a key contributor to high-fat diet (HFD)-related diseases, such as type 2 diabetes, non-alcoholic steatohepatitis, and atherosclerosis. The inflammation is characterized by infiltration of inflammatory cells, particularly macrophages, into obese adipose tissue. However, the molecular mechanisms by which a HFD induces low-grade inflammation are poorly understood. Here, we show that histone H3, a major protein component of chromatin, is released into the extracellular space when mice are fed a HFD or macrophages are stimulated with the saturated fatty acid palmitate. In a murine macrophage cell line, RAW 264.7, palmitate activated reactive oxygen species (ROS) production and JNK signaling. Inhibitors of these pathways dampened palmitate-induced histone H3 release, suggesting that the extracellular release of histone H3 was mediated, in part, through ROS and JNK signaling. Extracellular histone activated endothelial cells toexpress the adhesion molecules ICAM-1 and VCAM-1 and the procoagulant molecule tissue factor, which are known to contribute to inflammatory cell recruitment and thrombosis. These results suggest the possible contribution of extracellular histone to the pathogenesis of HFD-induced inflammation and thrombosis.},

  doi          = {10.1016/J.BBRC.2013.06.117},

  url          = {https://www.osti.gov/biblio/22239695},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 4,

  volume       = 437,

  place        = {United States},

  year         = {Fri Aug 09 00:00:00 EDT 2013},

  month        = {Fri Aug 09 00:00:00 EDT 2013}

}

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