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Title: High extensibility of stress fibers revealed by in vitro micromanipulation with fluorescence imaging

Abstract

Highlights: •We isolate contractile stress fibers from vascular smooth muscle cells. •We measure the extensibility of individual stress fibers. •We present the first direct evidence that individual stress fibers are highly extensible. •We quantitatively determine the local strain along the length of stress fibers. •The high extensibility we found is beyond that explained by a conventional model. -- Abstract: Stress fibers (SFs), subcellular bundles of actin and myosin filaments, are physically connected at their ends to cell adhesions. The intracellular force transmitted via SFs plays an essential role in cell adhesion regulation and downstream signaling. However, biophysical properties intrinsic to individual SFs remain poorly understood partly because SFs are surrounded by other cytoplasmic components that restrict the deformation of the embedded materials. To characterize their inherent properties independent of other structural components, we isolated SFs from vascular smooth muscle cells and mechanically stretched them by in vitro manipulation while visualizing strain with fluorescent quantum dots attached along their length. SFs were elongated along their entire length, with the length being approximately 4-fold of the stress-free length. This surprisingly high extensibility was beyond that explained by the tandem connection of actin filaments and myosin II bipolar filaments present in SFs, thusmore » suggesting the involvement of other structural components in their passive biophysical properties.« less

Authors:
 [1];  [2];  [3]
  1. Department of Biomolecular Sciences, Tohoku University (Japan)
  2. Department of Biomedical Engineering, Tohoku University (Japan)
  3. Department of Bioengineering and Robotics, Tohoku University (Japan)
Publication Date:
OSTI Identifier:
22239583
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 434; Journal Issue: 3; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; BIOPHYSICS; FIBERS; FILAMENTS; FLUORESCENCE; IN VITRO; MUSCLES; MYOSIN; QUANTUM DOTS

Citation Formats

Matsui, Tsubasa S., Sato, Masaaki, Department of Bioengineering and Robotics, Tohoku University, and Deguchi, Shinji. High extensibility of stress fibers revealed by in vitro micromanipulation with fluorescence imaging. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.03.093.
Matsui, Tsubasa S., Sato, Masaaki, Department of Bioengineering and Robotics, Tohoku University, & Deguchi, Shinji. High extensibility of stress fibers revealed by in vitro micromanipulation with fluorescence imaging. United States. https://doi.org/10.1016/J.BBRC.2013.03.093
Matsui, Tsubasa S., Sato, Masaaki, Department of Bioengineering and Robotics, Tohoku University, and Deguchi, Shinji. 2013. "High extensibility of stress fibers revealed by in vitro micromanipulation with fluorescence imaging". United States. https://doi.org/10.1016/J.BBRC.2013.03.093.
@article{osti_22239583,
title = {High extensibility of stress fibers revealed by in vitro micromanipulation with fluorescence imaging},
author = {Matsui, Tsubasa S. and Sato, Masaaki and Department of Bioengineering and Robotics, Tohoku University and Deguchi, Shinji},
abstractNote = {Highlights: •We isolate contractile stress fibers from vascular smooth muscle cells. •We measure the extensibility of individual stress fibers. •We present the first direct evidence that individual stress fibers are highly extensible. •We quantitatively determine the local strain along the length of stress fibers. •The high extensibility we found is beyond that explained by a conventional model. -- Abstract: Stress fibers (SFs), subcellular bundles of actin and myosin filaments, are physically connected at their ends to cell adhesions. The intracellular force transmitted via SFs plays an essential role in cell adhesion regulation and downstream signaling. However, biophysical properties intrinsic to individual SFs remain poorly understood partly because SFs are surrounded by other cytoplasmic components that restrict the deformation of the embedded materials. To characterize their inherent properties independent of other structural components, we isolated SFs from vascular smooth muscle cells and mechanically stretched them by in vitro manipulation while visualizing strain with fluorescent quantum dots attached along their length. SFs were elongated along their entire length, with the length being approximately 4-fold of the stress-free length. This surprisingly high extensibility was beyond that explained by the tandem connection of actin filaments and myosin II bipolar filaments present in SFs, thus suggesting the involvement of other structural components in their passive biophysical properties.},
doi = {10.1016/J.BBRC.2013.03.093},
url = {https://www.osti.gov/biblio/22239583}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 434,
place = {United States},
year = {Fri May 10 00:00:00 EDT 2013},
month = {Fri May 10 00:00:00 EDT 2013}
}